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SCM005 ReNcell® NSC Maintenance Media

SCM005
500 mL  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSCM005
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionReNcell® NSC Maintenance Media
OverviewReNcell® NSC Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell® immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell® immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell® NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell® immortalized neural progenitor cells
Materials Required but Not Delivered1. ReNcell® CX Immortalized Cell Line (CHEMICON Cat. No. SCC007)
2. ReNcell® VM Immortalized Cell Line (CHEMICON Cat. No. SCC008)
3. Basic fibroblast growth factor (bFGF; FGF-2; Specific Activity greater than 2 X 106 Units/mg. CHEMICON Cat. No. GF003)
4. Epidermal growth factor (EGF; Specific Activity greater than 1 x 107 Units/mg; CHEMICON Cat. No. GF001) 5. Laminin (Sigma Cat. No. L-2020)
5. Accutase (CHEMICON Cat. No. SCR005)
6. Tissue culture-ware
7. Phosphate-Buffered Saline (1X PBS) (CHEMICON Cat. No. BSS-1005-B)
8. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
9. Blocking Solution (5 percent normal donkey serum, 0.3 % Triton X-100 in 1X PBS)
10. Primary and secondary antibodies
Product Information
PresentationReNcell® NSC Maintenance Medium contains DMEM/F12, HEPES, L-glutamine, human serum albumin, human transferrin, putrescine dihydrochloride, human recombinant insulin, L-thyroxine, Tri-iodo-thyronine, progesterone, sodium selenite, heparin, corticosterone. The media does not contain antibiotics.
Quality LevelMQ100
Applications
ApplicationReNcell® NSC Maintenance Medium is a defined serum-free, growth factor-free neural stem cell medium that has been optimized for the growth & in vitro differentiation of ReNcell® immortalized human neural progenitor cells.
Key Applications
  • Stem Cell Culture
Biological Information
Media FormLiquid
Stem Cell Type
  • Neural Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsSCM005, ReNcell® Neural Stem Cell Maintenance Medium should be stored at -20°C until ready to use. Upon thawing, the maintenance medium should be stored at 2-8°C and given a 1-month expiration dating.
Packaging Information
Material Size500 mL
Global Trade Item Number
Catalogue Number GTIN
SCM005 04053252466205