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17-300 Ral Activation Assay Kit

17-300
1 kit  Kit capacity: 30 assays
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key Applications
ABA
Description
Catalogue Number17-300
Brand Family Upstate
Trade Name
  • Upstate
DescriptionRal Activation Assay Kit
Product Information
Components
  • Ral Activation Assay Buffer, 5X (Cat.# 20-196)
  • 100X GTPγS, 10mM (Cat.# 20-176)
  • 100X GDP, 100mM (Cat.# 20-177)
  • Anti-Ral-A
  • Ral Assay Reagent (Ral BP1, agarose) (Cat.# 14-415)
Quality LevelMQ100
Applications
ApplicationNon-radioactive Ral Activation Assay Kit can be used to precipitate Ral-A-GTP from cell lysates & detection by a Ral-A specific antibody.
Key Applications
  • Affinity Binding Assay
Biological Information
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors.
Gene Symbol
  • RALA
  • RAL
  • MGC48949
UniProt Number
UniProt SummarySIZE: 206 amino acids; 23567 Da
SUBUNIT: Interacts with RALBP1 via its effector domain. Interacts with EXOC8 and EXOC2. EXOC2 and EXOC8 have overlapping binding sites and compete for RALA binding. Interacts with Clostridium exoenzyme C3.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
SIMILARITY: SwissProt: P11233 ## Belongs to the small GTPase superfamily. Ras family.
Product Usage Statements
Quality AssuranceRoutinely evaluated by affinity precipitation/immunoblot analysis. Ral-A-GTP was precipitated from EGF-stimulated Cos-7 cell lysates. The precipitated Ral-A-GTP was detected by Western immunoblot analysis using anti-Ral A.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size1 kit
Material PackageKit capacity: 30 assays
Global Trade Item Number
Catalogue Number GTIN
17-300 04053252622861