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553506 RAP, Human, Recombinant, E. coli

553506
Purchase on Sigma-Aldrich

Overview

Products

Catalogue NumberPackaging Qty/Pack
553506-50UG Plastic ampoule 50 μg
Description
OverviewRecombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.
Catalogue Number553506
Brand Family Calbiochem®
SynonymsReceptor Associated Protein
References
ReferencesLi, Y., et al. 2002. Biochemistry 41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
Bu, G. 2001. Int. Rev. Cytol. 209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035
Product Information
FormLiquid
Formulation150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
Quality LevelMQ100
Biological Information
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
553506-50UG 04055977193664