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444810 ProteoExtract® Native Membrane Protein Extraction Kit

444810
Purchase on Sigma-Aldrich

Overview

Products

Catalogue NumberPackaging Qty/Pack
444810-1KIT Glass bottle 1 kit
Description
OverviewA convenient kit for the isolation of native integral membrane and membrane-associated proteins, without the need for ultracentrifugation. Extraction is based on association of proteins with cellular membranes rather than on their hydrophobicity. Resulting samples are suitable for use in functional assays, 2-D gel electrophoresis, and other applications.
Note: 1 kit is sufficient for up to 20 sample extractions.
Catalogue Number444810
Brand Family Calbiochem®
SynonymsM-PEK Kit
Materials Required but Not Delivered Platform mixer e.g., IKA Vibramax (when extracting adherent tissue culture cells)
Cell culture equipment, media etc. for cell growth (e. g., RPMI, DMEM).
Micropipettes and tips, 10 µl, 200 µl and 1 ml size (e.g., Eppendorf, Gilson or equivalent)
Cooled centrifuge and rotor for 50 or 15 ml tube size (Eppendorf, Heraeus, Nalgene, etc.)
Cooled micro centrifuge and rotor up to 18,000 x g for 2 ml tube size (e.g. Eppendorf)
Thermo mixer or rolling facility (e.g., Eppendorf)
Homogenizer (e.g. potter or Dounce) or mortar.
Product Information
Form20 Extractions
Kit containsWash Buffer, Extraction Buffer 1, Extraction Buffer 2, Protease Inhibitor Cocktail, and a user protocol.
Biological Information
Sample TypeMammalian tissues and cultured cells
Product Usage Statements
Intended useThe ProteoExtract™ Native Membrane Protein Extraction Kit is designed for the isolation of native membrane proteins from a broad range of mammalian samples, including:
• Adherent tissue culture cells
• Suspension grown tissue culture cells
• Frozen cell pellets
• Tissues
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +2°C to +8°C
Storage Conditions• Wash Buffer
Store at 4°C.
• Extraction Buffers I & II
The Extraction Buffers I and II can be stored at 4°C for up to 6 month.
For prolonged storage, freeze the buffers in convenient aliquots at -20°C. Before extraction, buffers must be thawed at room temperature (RT). After thawing, mix components by gently shaking or vortexing. Avoid repeated freezing and thawing!
• Protease Inhibitor Cocktail
The Protease Inhibitor Cocktail is supplied in DMSO and can be stored at 4°C up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C. During the sample preparation procedure it must be kept at RT to prevent freezing of DMSO.
Do not freeze Ok to freeze
Supplemental Information
Kit containsWash Buffer, Extraction Buffer 1, Extraction Buffer 2, Protease Inhibitor Cocktail, and a user protocol.
Global Trade Item Number
Catalogue Number GTIN
444810-1KIT 07790788055653