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DR1025 PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb

This PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
DR1025
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, Mk, M, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DR1025-50UL Plastic ampoule 50 ul
Description
OverviewRecognizes the ~56 kDa Chk1 protein phosphorylated at Ser317 in UV-treated COS cells.
Catalogue NumberDR1025
Brand Family Calbiochem®
Application Data
Detection of human and monkey Chk1, phosphorylated at Ser317, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer317) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.
References
ReferencesZhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev. 14, 289.
Martinho, R.G., et al. 1998. EMBO J. 17, 7239.
Zeng, Y., et al. 1998. Nature 395, 507.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Positive controlLysates from COS cells treated with UV
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesImmunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50)
Application CommentsDoes not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Monkey
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsDo not aliquot.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1025-50UL 04055977225914

Documentation

PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb Certificates of Analysis

TitleLot Number
DR1025

References

Reference overview
Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev. 14, 289.
Martinho, R.G., et al. 1998. EMBO J. 17, 7239.
Zeng, Y., et al. 1998. Nature 395, 507.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-August-2007 RFH
ApplicationImmunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50)
Application Data
Detection of human and monkey Chk1, phosphorylated at Ser317, by immunoblotting. Samples: Whole cell lysate from HeLa cells treated with hydroxyurea (Cat. No. 400046) or COS cells treated with hydroxyurea or UV for the indicated times. Primary antibody: PhosphoDetect™ Anti-Chk1 (pSer317) Rabbit pAb (Cat. No. DR1025) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser317.
BackgroundChk1 is a downstream protein kinase of ATM/ATR that plays an improtant role in DNA damage checkpoint, development, and tumor suppression. Chk1 is activated in response to various forms of genotoxic stress and replication blocks. Chk1 is activated by phosphorylation on Ser317 and Ser345. Chk1 can inactivate cdc25c and phosphorylate p53.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
IsotypeIgG
Specieshuman, monkey, mouse, rat
Positive controlLysates from COS cells treated with UV
FormLiquid
FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
PreservativeNone
CommentsDoes not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsDo not aliquot.
Toxicity Standard Handling
ReferencesZhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol. 21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev. 14, 289.
Martinho, R.G., et al. 1998. EMBO J. 17, 7239.
Zeng, Y., et al. 1998. Nature 395, 507.