Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

524400 Phorbol-12-myristate-13-acetate - CAS 16561-29-8 - Calbiochem

Phorbol-12-myristate-13-acetate, CAS 16561-29-8, acts as an extremely potent mouse skin tumor promoter. Activates protein kinase C in vivo and in vitro at nM concentrations.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: PMA, TPA, PKC Activator I

Primary Target: Protein Kinase C
524400
Purchase on Sigma-Aldrich

Overview

Key Spec Table

CAS #Empirical Formula
16561-29-8C₃₆H₅₆O₈

Products

Catalogue NumberPackaging Qty/Pack
524400-10MG Glass bottle 10 mg
Add To Favorites
524400-1MG Glass bottle 1 mg
Add To Favorites
524400-25MG Glass bottle 25 mg
Add To Favorites
524400-5MG Glass bottle 5 mg
Add To Favorites
Description
OverviewMost commonly-used phorbol ester. Extremely potent mouse skin tumor promoter. Activates protein kinase C in vivo and in vitro, even at nM concentrations. Promotes the expression of inducible NOS in cultured hepatocytes. Activates Ca2+-ATPase and potentiates forskolin-induced cAMP formation. Inhibits apoptosis induced by the Fas antigen, but induces apoptosis in HL-60 promyelocytic leukemia cells. Its binding is reversible. Also available as a 10 mM solution in DMSO (Cat. No. 500582).
Catalogue Number524400
Brand Family Calbiochem®
SynonymsPMA, TPA, PKC Activator I
References
ReferencesPowell, D.J., et al. 2003. Mol. Cell Biol. 23, 7794.
Tepper, C.G., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8443.
Oishi, K., and Yamaguchi, M. 1994. J. Cell. Biochem. 55, 168.
Macfarlane, D.E., and O’Donnell, P.S. 1993. Leukemia 7, 1846.
Ghandi, V.C. and Jones, D.J., 1992. Neuropharmacol. 31, 1101.
Hortelano, S., et al. 1992. J. Biol. Chem. 267, 24937.
Kontny, E., et al. 1992. Eur. J. Pharmacol. 227, 333.
Zanaboni, P.B., et al. 1992. J. Appl. Physiol. 73, 2011.
Saltis, J., et al. 1991. J. Biol. Chem. 266, 261.
Beguinot, L., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 2774.
Perchellet, J. 1985. Carcinogenesis 6, 567.
Nishizuka, Y. 1984. Science 255, 1365.
Mastro, A. 1982. Lymphokines 6, 263.
Product Information
CAS number16561-29-8
ATP CompetitiveN
FormWhite solid
Hill FormulaC₃₆H₅₆O₈
Chemical formulaC₃₆H₅₆O₈
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationPhorbol-12-myristate-13-acetate, CAS 16561-29-8, acts as an extremely potent mouse skin tumor promoter. Activates protein kinase C in vivo and in vitro at nM concentrations.
Biological Information
Primary TargetProtein Kinase C
Purity≥98% by HPLC
Physicochemical Information
Cell permeableN
Safety Information according to GHS
RTECSQH4377000
Safety Information
R PhraseR: 21-38

Harmful in contact with skin.
Irritating to skin.
S PhraseS: 26-27-36-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Take off immediately all contaminated clothing.
Wear suitable protective clothing.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Anhydrous DMSO stock solutions are stable for up to 6 months at -20°C. With frequent use, the DMSO stock solutions may accumulate water. The presence of water in the stock solution will accelerate the decomposition of the product, resulting in significant loss of activity in 2-3 months.
Packaging Information
Packaged under inert gas Packaged under inert gas
Global Trade Item Number
Catalogue Number GTIN
524400-10MG 04055977270952
524400-1MG 04055977270945
524400-25MG 04055977270969
524400-5MG 04055977270976