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MAB3242-KC Mouse Anti-Smoothelin, clone R4A (1mg) KC

Detect Smoothelin using this Anti-Smoothelin Antibody, clone R4A validated for use in WB, IC, IH(P).

Mouse Anti-Smoothelin, clone R4A (1mg) KC MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
MAB3242-KC
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Description
Catalogue NumberMAB3242-KC
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionMouse Anti-Smoothelin, clone R4A (1mg) KC
OverviewThis product is intended for use by IVD manufacturers.
Contact customer service for assistance: oem.reagents@emdmillipore.com
Alternate Names
  • Anti-Smoothelin
Background InformationSmoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cell with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin or vimentin. Smoothelin colocalizes with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 50 kDa isoform specific for visceral SMC, and an isoform with a molecular weight of approximately 100 kDa in vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.MAB3242 is useful to discriminate fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.
References
Product Information
FormatPurified
PresentationLiquid in buffer (PBS) with 0.1% sodium azide.
Quality LevelMQ300
Applications
ApplicationDetect Smoothelin using this Anti-Smoothelin Antibody, clone R4A validated for use in WB, IC, IH(P).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application NotesWestern blot

Immunocytochemistry

Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenCytoskeletal extract of chicken gizzard.
CloneR4A
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityMAB3242 reacts with smoothelin, a cytoskeletal protein exclusively found in smooth muscle cells. Reacts with the 59 kDa and 100 kDa protein corresponding to smoothelin by Western blot.
IsotypeIgG1
Species Reactivity
  • Canine
  • Chicken
  • Human
  • Hamster
  • Monkey
  • Pig
  • Feline
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in three transcript variants.
Gene Symbol
  • SMTN
  • smoothelin
  • Smoothelin.
  • FLJ38597
  • SMSMO
  • FLJ35365
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P53814 # Structural protein (cytoskeletal).
SIZE: 917 amino acids; 99516 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Exhibits a filamentous organization.
TISSUE SPECIFICITY: Smooth muscle; contractile or vascular (for the long form).
SIMILARITY: SwissProt: P53814 ## Contains 1 CH (calponin-homology) domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material SizeBulk packaging. Please inquire.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3242-KC 04053252683107

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Catalogue Number Description
MAB3242 Anti-Smoothelin Antibody, clone R4A

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies
IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Muscular Dystrophy related
IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Secondary Bulk Antibodies > Anti-Human Antibodies & Conjugates > Mouse Anti-Human
IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Other Primary Bulk Antibodies