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FCMAB186F Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1

FCMAB186F
100 tests  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMFITCMonoclonal Antibody
Description
Catalogue NumberFCMAB186F
Trade Name
  • Milli-Mark
DescriptionMilli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1
Alternate Names
  • Membrane-spanning 4-domains subfamily A member 1
    B-lymphocyte surface antigen B1
    Leukocyte surface antigen Leu-16
    Bp35
    CD_antigen=CD20
Background InformationCD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells (Tedder, 1994). In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells and B-cell lines (Zhou, 1995). The long N- and C-terminal ends of the protein are located on the cytoplasmic side of the membrane and only a minor portion of the protein is exposed on the cell surface (Tedder, 1994). Antibodies reacting with CD20 cytoplasmic epitopes are designated CD20cy (Zhou, 1995). It is suggested that CD20 plays a direct role in regulating the transmembrane conductive Ca 2+ flux of B cells which indicates a possible function for CD20 as a regulator of proliferation and differentiation (Tedder, 1994).
Product Information
FormatFITC
Control
  • Human Lymphocytes
PresentationPurified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2
Quality LevelMQ100
Applications
ApplicationThis Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1 is validated for use in FC for the detection of CD20.
Key Applications
  • Flow Cytometry
Biological Information
CloneB-Ly1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityAntibody recognizes Human CD20 antigen.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight 33 kDa (Zhou, 1995)
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using human lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Global Trade Item Number
Catalogue Number GTIN
FCMAB186F 04053252289149