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14-779 MKP5, active

MKP5, active MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-779
10 µg  Also available in 250μg size as catalog # 14-779M and in bulk as catalog # 14-779-K.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-779
Trade Name
  • Upstate
DescriptionMKP5, active
OverviewDual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the
phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily
(MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members ofthis family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue
distribution and subcellular localization, and different modes of inducibility of their expression by extracellular
stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization
is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various
tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different
isoforms have been found for this gene.
References
Product Information
PresentationProtein in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ100
Applications
Key Applications
  • Phosphatase Assay
Biological Information
SourceN-terminal 6His-tagged, recombinant, human MKP5, amino acids 320-end, expressed in E. coli.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • DUSP10
  • MKP5
Purification MethodPurified using Ni2+/NTA agarose.
UniProt Number
Molecular Weight20.6 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by the release of phosphate from the substrate 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size as catalog # 14-779M and in bulk as catalog # 14-779-K.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-779 04053252365980

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-779M MKP5, active

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Phosphatases
Life Science Research > Proteins and Enzymes > Purified Phosphatases