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TGFBMAG-64K-03 MILLIPLEX MAP TGFß Magnetic Bead 3 Plex Kit - Immunology Multiplex Assay

This MILLIPLEX MAP TGFß (Transforming Growth Factor Beta) - 3 Plex is an Immunology Multiplex Assay is used for the simultaneous quantification of TGFß1, TGFß2 and TGFß3 in the following species: Mous

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology Research Sub-Category: Cardiovascular Disease
TGFBMAG-64K-03
96-well plate  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
TGF-β1 TGF-β2 TGF-β3 H, M, R, NhP, Ca, Fe, Po, EqMplexLuminex xMAP
Description
Catalogue NumberTGFBMAG-64K-03
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP TGFß Magnetic Bead 3 Plex Kit - Immunology Multiplex Assay
Background InformationThe transforming growth factor beta (TGFβ) system, a superfamily of cytokines as well as signaling pathways, is highly conserved throughout the animal kingdom. TGFβ functions in immune cell development, angiogenesis, wound healing and embryonic development, bone development and plays a critical role in immune response, heart disease, and cancer. In its normal state TGFβ is one of the few classes of proteins able to inhibit cell growth by halting mitosis at the G1 state, inducing cell differentiation or apoptosis. However, during oncogenesis mutations in the TGFβ signaling pathway result in tumor cell resistance to the effects of normally functioning TGFβ, causing proliferation without regulation. Initial research suggests that VE-cadherin may enhance the mutated TGFβ signaling pathway.
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Inter-assay: < 10
  • Intra-assay: < 10
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationThis MILLIPLEX MAP TGFß (Transforming Growth Factor Beta) - 3 Plex is an Immunology Multiplex Assay is used for the simultaneous quantification of TGFß1, TGFß2 and TGFß3 in the following species: Mouse/Human/Rat/Pig/Horse/Dog/Cat/Non-Human Primate.
Key Applications
  • Multiplexing
Application Notes• This is a 2-hour or overnight incubation assay.
• This assay requires 25 μL of a 1:30 dilution after treatment of serum and plasma samples.
• In addition to human, TGFβ1 was detected in acid-activated serum of every species tested which included horse, rabbit, mouse, rat, mini pig, canine, hamster, guinea pig, rhesus monkey and cynomolgous monkey. In addition to human, TGFβ2 was detected in the acid-activated serum of the following: horse, rabbit, mouse, rat, mini pig, canine, hamster, guinea pig, rhesus monkey and cynomolgous monkey. In addition to human, TGFβ3 was detected in mouse, rat, mini pig, hamster, guinea pig, rhesus monkey and cynomolgous monkey samples.

However, the exact amount of cross reactivity has not been determined.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Non-human Primate
  • Canine
  • Feline
  • Pig
  • Horse
Analytes Available
  • TGF-β1
  • TGF-β2
  • TGF-β3
Assay PanelPremixed beads for your convenience.
Accuracy
  • 62% - 128%
Physicochemical Information
Sensitivity
  • 8 - 12 pg/mL
Standard Curve Range
  • 9.8 - 2,500 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Global Trade Item Number
Catalogue Number GTIN
TGFBMAG-64K-03 04055977289183