Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

GF342 LIF,
Human Recombinant Animal Free

LIF,
Human Recombinant Animal Free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P15018
GF342
25µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SourcePurity
Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.		Product endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
Description
Catalogue NumberGF342
DescriptionLIF,
Human Recombinant Animal Free
Alternate Names
  • Leukemia Inhibitory Factor
  • Emfilermin
  • MLPLI
  • HILDA
  • D-FACTOR
Background InformationLIF is a pleiotrophic factor produced by multiple cell types including T cells, myelomoncytic lineages, fibroblasts, liver, heart and melanoma. LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While human LIF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LIF is not active on human cells due to its inability to bind to the human LIF receptor.
Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds.
References
Product Information
PresentationProduct is presented without additives and is filtered through a 0.2 micron filter before lyophilization.
Quality LevelMQ100
Applications
Biological Information
PurityProduct endotoxin level is <0.1 ng/μg of protein (<1 EU/μg).
SourceProtein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceDetermined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤0.1 ng/ml, corresponding to a specific activity of ≥1 x 10^7 units/mg.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
Packaging Information
Material Size25µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF342 04053252978968

Documentation

LIF,
Human Recombinant Animal Free SDS

Title

Safety Data Sheet (SDS) 

LIF,
Human Recombinant Animal Free Certificates of Analysis

TitleLot Number
LIF, Human Recombinant Animal Free - 2468399 2468399
LIF, Human Recombinant Animal Free - Q2436473 Q2436473
LIF, -2644001 2644001
LIF, -2714456 2714456
LIF, -2728266 2728266
LIF, Human Recombinant Animal Free - 3191423 3191423
LIF, Human Recombinant Animal Free - 3236380 3236380
LIF, Human Recombinant Animal Free - 3243481 3243481
LIF, Human Recombinant Animal Free - 3385865 3385865
LIF, Human Recombinant Animal Free - 3752730 3752730
Show more

Related Products & Applications

Product Families

Cytokines and Growth Factors

EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in human, mouse, and rat cell culture, stem cell differentiation and ELISA studies.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors