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420298 Sigma-AldrichK-252a, Nocardiopsis sp. - CAS 97161-97-2 - Calbiochem

K-252a, Nocardiopsis sp., CAS 97161-97-2, is a cell-permeable, ATP-competitive inhibitor of multiple protein kinases (Ki = 1.8, 20, 18, 25, 20, 3 nM for CaMKII, MLCK, PKA, PKC, PKG & 140 trk).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet
Citations

Primary Target: CaM kinase II

Recommended Products

Overview

Replacement Information

Key Spec Table

CAS #	Empirical Formula
97161-97-2	C₂₇H₂₁N₃O₅

Products

Catalogue Number	Packaging	Qty/Pack
420298-100UG	Plastic ampoule	100 μg	Add To Favorites Add To Favorites Added To My Favorites

Description
Overview	A cell-permeable, reversible, and ATP-competitive inhibitor against multiple protein kinases, including, but not limited to, CaM kinase II (K_i = 1.8 nM), myosin light chain kinase (K_i = 20 nM), protein kinase A (K_i = 18 nM), protein kinase C (K_i = 25 nM), protein kinase G (K_i = 20 nM), NGF receptor gp140^trk (IC₅₀ = 3 nM), its transforming trk oncogenes, and the related neurotrophin receptors gp145^trkB and gp145^trkC. Reported to induce apoptosis and cell cycle arrest by decreasing Cdk1 and Cdc25c activity in T98G cells and inhibit Flt3 phosphorylation in Flt3/Itd-BaF3 cells (IC₅₀ <50 nM). Also available as a premade 1 mM (100 µg/214 µl) solution in DMSO (Cat. No. 420297).
Catalogue Number	420298
Brand Family	Calbiochem®

References
References	Cools, J., et al. 2004. Cancer Res. 64, 6385. Chin, L.S., et al. 1999. Cancer Invest. 17, 391. Tapley, P., et al. 1992. Oncogene 7, 371. Hashimoto, Y. 1991. Biochem. Biophys. Res. Commun. 181, 423. Nakanishi, S. 1988. J. Biol. Chem. 263, 6215. Kase, H., et al. 1987. Biochem. Biophys. Res. Commun. 142, 436.

Product Information
CAS number	97161-97-2
ATP Competitive	Y
Form	White solid
Hill Formula	C₂₇H₂₁N₃O₅
Chemical formula	C₂₇H₂₁N₃O₅
Reversible	Y
Structure formula Image
Quality Level	MQ100

Applications
Application	K-252a, Nocardiopsis sp., CAS 97161-97-2, is a cell-permeable, ATP-competitive inhibitor of multiple protein kinases (Ki = 1.8, 20, 18, 25, 20, 3 nM for CaMKII, MLCK, PKA, PKC, PKG & 140 trk).

Biological Information
Primary Target	CaM kinase II
Primary Target K<sub>i</sub>	1.8 nM, 20 nM, 18 nM, 25 nM, 20 nM, 3 nM, against CaM kinase II, myosin light chain kinase, protein kinase A, protein kinase C, protein kinase G, NGF receptor gp140trk, respectively
Purity	≥95% by HPLC

Physicochemical Information
Cell permeable	Y

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
RTECS	NZ0550000

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
420298-100UG	04055977210910

Documentation

K-252a, Nocardiopsis sp. - CAS 97161-97-2 - Calbiochem SDS

Title
Safety Data Sheet (SDS)

K-252a, Nocardiopsis sp. - CAS 97161-97-2 - Calbiochem Certificates of Analysis

Title	Lot Number
420298	Enter Lot Number

References

Reference overview
Cools, J., et al. 2004. Cancer Res. 64, 6385. Chin, L.S., et al. 1999. Cancer Invest. 17, 391. Tapley, P., et al. 1992. Oncogene 7, 371. Hashimoto, Y. 1991. Biochem. Biophys. Res. Commun. 181, 423. Nakanishi, S. 1988. J. Biol. Chem. 263, 6215. Kase, H., et al. 1987. Biochem. Biophys. Res. Commun. 142, 436.

Citations

Title

Huanyu Dou, et al. (2005) Neuroprotective mechanisms of lithium in murine human immunodeficiency virus-1 encephalitis. Journal of Neuroscience 25, 8375-8385.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	25-November-2009 RFH
Description	A cell-permeable, reversible, and ATP-competitive inhibitor against multiple protein kinases, including, but not limited to, CaM kinase II (K_i = 1.8 nM), myosin light chain kinase (K_i = 20 nM), protein kinase A (K_i = 18 nM), protein kinase C (K_i = 25 nM), protein kinase G (K_i = 20 nM), NGF receptor gp140^trk (IC₅₀ = 3 nM), its transforming trk oncogenes, and the related neurotrophin receptors gp145^trkB and gp145^trkC. Reported to induce apoptosis and cell cycle arrest by decreasing Cdk1 and Cdc25c activity in T98G cells and inhibit Flt3 phosphorylation in Flt3/Itd-BaF3 cells (IC₅₀ <50 nM). Also available as a premade 1 mM (100 µg/214 µl) solution in DMSO (Cat. No. 420297).
Form	White solid
CAS number	97161-97-2
RTECS	NZ0550000
Chemical formula	C₂₇H₂₁N₃O₅
Structure formula
Purity	≥95% by HPLC
Solubility	DMF or DMSO (1 mg/ml)
Storage	Protect from light -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity	Standard Handling
References	Cools, J., et al. 2004. Cancer Res. 64, 6385. Chin, L.S., et al. 1999. Cancer Invest. 17, 391. Tapley, P., et al. 1992. Oncogene 7, 371. Hashimoto, Y. 1991. Biochem. Biophys. Res. Commun. 181, 423. Nakanishi, S. 1988. J. Biol. Chem. 263, 6215. Kase, H., et al. 1987. Biochem. Biophys. Res. Commun. 142, 436.
Citation	Huanyu Dou, et al. (2005) Neuroprotective mechanisms of lithium in murine human immunodeficiency virus-1 encephalitis. Journal of Neuroscience 25, 8375-8385.

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