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IL035 Interleukin-10 Protein, Recombinant rat

The recombinant rat Interleukin-10 protein, originally known as Cytokine Synthesis Inhibitory Factor (CSIF) is an 18.7 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI.

Interleukin-10 Protein, Recombinant rat MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P15248, P29456
IL035
10 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSpeciesPurity
CULTRat Greater than 98% by SDS-PAGE and HPLC analysis
Description
Catalogue NumberIL035
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionInterleukin-10 Protein, Recombinant rat
OverviewRecombinant Rat Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory factor (CSIF), is an 18.7 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI.
Alternate Names
  • IL-10
References
Product Information
PresentationLyophilized from 5mM Tris, pH 7.2.
Quality LevelMQ100
Applications
ApplicationThe recombinant rat Interleukin-10 protein, originally known as Cytokine Synthesis Inhibitory Factor (CSIF) is an 18.7 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI.
Key Applications
  • Cell Culture
Biological Information
PurityGreater than 98% by SDS-PAGE and HPLC analysis
Specific ActivityThe ED(50) was determined by the dose-dependent inhibition of antigen-specific T-cell proliferation is <10 ng/mL.
Entrez Gene SummaryThe protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P15248 # Supports IL-2 independent and IL-4 independent growth of helper T-cells.
SIZE: 144 amino acids; 15909 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P15248 ## Belongs to the IL-7/IL-9 family.
Stem Cell Type
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20ºC. We recommend a quick spin followed by reconstitution in 5 mM Tris, pH7.2, to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or -20ºC for future use. Reconstituted IL-10 should be stored in working aliquots at -20°C.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IL035 04053252408731

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Categories

Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors