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71187 Insect PopCulture® Reagent

Protein extraction directly from insect cell cultures

Insect PopCulture® Reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71187
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
71187-3 Plastic ampoule 50 ml
71187-4 Plastic ampoule 250 ml

Insect PopCulture® Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His•Bind® affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.



Description

Catalogue Number71187 Brand Family Novagen®
Features and benefits
  • No need to separate cells from culture media
  • No need to clarify cell extracts prior to purification
  • Higher protein yield due to target protein recovery from both medium and cells
  • Direct affinity adsorption of target proteins to IMAC affinity resin from the total culture extract
  • Compatible with protease inhibitor cocktails
  • Ideal for high-throughput, expression-level screening and protein purification
  • Compatible with transfected and baculovirus-infected insect cell cultures
References
ReferencesLoomis, K., et al. 2002. inNovations 15, 16.
Product Information
DeclarationThis product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71187-3 04055977254075
71187-4 04055977254082