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14-684M IRAK1 Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant, human IRAK1 amino acids 194-end. For use in Kinase Assays.

IRAK1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-684M
250 µg  Also available in 10 μg size (cat#14-684) and in bulk (cat# 14-684-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-684M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionIRAK1 Protein, active, 250 µg
OverviewN-terminal 6His-tagged, recombinant, human IRAK1 amino acids 194-end.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human IRAK1 amino acids 194-end. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman IRAK1 expressed by baculovirus in Sf21 cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • IRAK1
  • IRAK
  • IRAK-1
  • pelle
Protein TargetIRAK1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTKL
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P51617 # Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
COFACTOR: Magnesium.
SIZE: 712 amino acids; 76537 Da
SUBUNIT: IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Interacts with IL1RL1.
TISSUE SPECIFICITY: Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
PTM: Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
SIMILARITY: SwissProt: P51617 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. & Contains 1 protein kinase domain.
Molecular Weight59.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of MBP (13-104).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-684) and in bulk (cat# 14-684-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-684M 04053252732300

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Alternative Packsize

Catalogue Number Description
14-684 IRAK1 Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases