Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

GF446-MG HumanKine® Thermostable bFGF,
Human Recombinant

Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P09038
GF446-MG
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SourcePurity
HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.Product is endotoxin free (<0.5 ng/μg of protein (<5 EU/μg) and greater than 95% pure by SDS-PAGE.
Description
Catalogue NumberGF446-MG
Trade Name
  • HumanKine®
DescriptionHumanKine® Thermostable bFGF,
Human Recombinant
Alternate Names
  • FGF2
  • prostatropin
  • Prostatropin
  • HBGF-2
  • BFGF
Background InformationThe HumanKine Thermostable bFGF is engineered with enchanced thermostability compared to the wild-type bFGF protein without modification of biological function.

Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions.
References
Product Information
PresentationLyophilized from a 0.2 μm filtered solution of 1X PBS.
Quality LevelMQ100
Applications
ApplicationEngineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.
Application NotesHumanKine products are Xeno-Free
Biological Information
PurityProduct is endotoxin free (<0.5 ng/μg of protein (<5 EU/μg) and greater than 95% pure by SDS-PAGE.
SourceHumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceBioactivity assay: Determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line. EC50 is typically ≤0.5 ng/mL
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF446-MG 04053252976056

Documentation

HumanKine® Thermostable bFGF,
Human Recombinant SDS

Title

Safety Data Sheet (SDS) 

HumanKine® Thermostable bFGF,
Human Recombinant Certificates of Analysis

TitleLot Number
HumanKine® Thermostable bFGF, -VP1410077 VP1410077
HumanKine® Thermostable bFGF, -VP1605042 VP1605042
HumanKine® Thermostable bFGF, -VP1606077 VP1606077
HumanKine® Thermostable bFGF, Human Recombinant - VP2207137 VP2207137
HumanKine® Thermostable bFGF, Human Recombinant - VP2412100 VP2412100
HumanKine® Thermostable bFGF, Human Recombinant -VP1510288 VP1510288

Technical Info

Title
HumanKine®Thermostable bFGF