Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

SCT150 Firefly Luciferase HTS Assay

Glow-type luciferase assay for the quantification of firefly luciferase gene expression in mammalian cells with signal half-life of about 3 hours

SCT150
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT150
DescriptionFirefly Luciferase HTS Assay
OverviewFirefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Firefly luciferase is a 62,000 Dalton protein, which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin, producing light emission centered at 560 nm. Light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules.

However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. The Firefly Luciferase HTS Assay is designed for is a proprietary mixture of substances that modify the enzymatic reaction to produce a long-lasting signal (steady-glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a homogeneous high sensitivity firefly luciferase reporter gene assay kit for the quantification of firefly luciferase expression in mammalian cells with signal half-life of about 3 hours (Figure 2). Glow-type luciferase assays have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.
Alternate Names
  • Firefly Luciferase Assay
  • Bioluminescent Assay
References
Product Information
Components
  • 1) Assay Buffer (CS224524): 1X100 ml
  • 2) D-Luciferin (CS224523): 1 X 25 mg
Detection methodChemiluminescence
PresentationLiquid
Applications
ApplicationGlow-type luciferase assay for the quantification of firefly luciferase gene expression in mammalian cells with signal half-life of about 3 hours
Key Applications
  • Cell Based Assays
Application NotesFirefly Luciferase Assay
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore Firefly Luciferase HTS Assay at -80°C. Kit components are stable for at least six months from date of receipt when stored as recommended. Avoid repeated freeze-thaw cycles.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT150 04054839566028