324725 Sigma-AldrichEndoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli
Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins.
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Synonyms: Endo-β-N-acetylglucosaminidase F1, Endo F1
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| Catalogue Number | Packaging | Qty/Pack | |
|---|---|---|---|
| 324725-700MIU | Glass bottle | 700 miu |
| Description | |
|---|---|
| Overview | Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins. |
| Note: 1 mU = 1 milliunit. | |
| Catalogue Number | 324725 |
| Brand Family | Calbiochem® |
| Synonyms | Endo-β-N-acetylglucosaminidase F1, Endo F1 |
| Product Information | |
|---|---|
| Activity | ≥17 units/ml |
| Unit of Definition | One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. |
| EC number | 3.2.1.96 |
| Form | Liquid |
| Formulation | In 20 mM Tris-HCl, pH 7.5. |
| Quality Level | MQ100 |
| Biological Information | |
|---|---|
| Specific Activity | ≥16 units/mg protein |
| Physicochemical Information | |
|---|---|
| Contaminants | Proteases: none detected. |
| Storage and Shipping Information | |
|---|---|
| Ship Code | Blue Ice Only |
| Toxicity | Standard Handling |
| Storage | +2°C to +8°C |
| Do not freeze | Yes |
| Global Trade Item Number | |
|---|---|
| Catalogue Number | GTIN |
| 324725-700MIU | 04055977215991 |