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251520 DAF-FM DA - CAS 254109-22-3 - Calbiochem

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

DAF-FM DA - CAS 254109-22-3 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate

251520
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
254109-22-3C₂₅H₁₈F₂N₂O₇

Products

Catalogue NumberPackaging Qty/Pack
251520-1MG Glass bottle 1 mg
Description
OverviewA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
Catalogue Number251520
Brand Family Calbiochem®
Synonyms3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
References
ReferencesKojima, H., et al. 2001. J. Neurochem. 76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.
Product Information
CAS number254109-22-3
ATP CompetitiveN
DeclarationManufactured by Daiichi Pure Chemicals Co., Ltd
FormPale yellow solution
FormulationA 5 mM solution in DMSO.
Hill FormulaC₂₅H₁₈F₂N₂O₇
Chemical formulaC₂₅H₁₈F₂N₂O₇
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetNitric oxide (NO) fluorescent indicator
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 26

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
251520-1MG 04055977198515

Documentation

DAF-FM DA - CAS 254109-22-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

DAF-FM DA - CAS 254109-22-3 - Calbiochem Certificates of Analysis

TitleLot Number
251520

References

Reference overview
Kojima, H., et al. 2001. J. Neurochem. 76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-December-2018 JSW
Synonyms3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
DescriptionA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.
FormPale yellow solution
FormulationA 5 mM solution in DMSO.
Recommended reaction conditions
The reagent is provided at a 5 mM concentration. Prepare a 500-fold dilution in 0.1 M phosphate buffer, pH 7.4 before use. BSA and phenol red may affect fluorescence.
CAS number254109-22-3
Chemical formulaC₂₅H₁₈F₂N₂O₇
Structure formulaStructure formula
Purity≥98% by HPLC
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
Toxicity Irritant
ReferencesKojima, H., et al. 2001. J. Neurochem. 76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed. 38, 3209.