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S7802 CpG WIZ® p15 -Methylation specific PCR assay

The CpG WIZ p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP).

CpG WIZ® p15 -Methylation specific PCR assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
S7802
25 assays  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberS7802
Brand Family Chemicon®
Trade Name
  • CpG Wiz
  • Chemicon
DescriptionCpG WIZ® p15 -Methylation specific PCR assay
OverviewAberrant methylation of the p15 promoter region has been shown to cause inactivation in several gliomas and leukemias. The CpG WIZ® p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unmethylated) for p15* are also included.



Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.



*p15 is covered under U.S. patents 5,994,095, US 6,140,473, 6,180,776, 6,210,949 and 6,218,146.
References
Product Information
Quality LevelMQ100
Applications
ApplicationThe CpG WIZ p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP).
Key Applications
  • PCR
Application NotesPrinciples of the Technique

Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the The CpG WIZ® p15 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.
Biological Information
Species Reactivity
  • Human
Entrez Gene Number
Entrez Gene SummaryThis gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
Gene Symbol
  • CDKN2B
  • TP15
  • INK4B
  • p15INK4b
  • p14-INK4b
  • P15
  • MTS2
  • p15-INK4b
  • MTS-2
  • p14_INK4B
  • p15INK4B
  • CDK4I
  • p15_INK4B
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42772 # Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
SIZE: 138 amino acids; 14722 Da
SUBUNIT: Heterodimer of CDKN2B with CDK4 or CDK6.
DISEASE:SwissProt: P42772 # Defects in CDKN2B are involved in tumor formation.
SIMILARITY: SwissProt: P42772 ## Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. & Contains 4 ANK repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size25 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
S7802 04053252726255