Centrifree® Ultrafiltration Device
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Overview
Specifications
Ordering Information
Documentation
References
| Reference overview | Application |
|---|---|
| Fractionation of canine serum magnesium. Schenck PA.,Vet Clin Pathol. 2005 Jun;34(2):137-9. Vet Clin Pathol. 2005 Jun;34(2):137-9. 2005 | |
| The indirect effect of Tityus discrepans on rabbit pulmonary vasculature. Novoa, Eva, et al. Respiratory physiology & neurobiology, 134: 33-41 (2003) 2003 Show Abstract | Cell Culture |
| Metabolic activity of cultured rat brainstem, hippocampal and spinal cord slices. Caroline A. Connelly, Lawrence C. Chen and Steven D. Colquhoun Journal of Neuroscience Methods 99 (1-2): 1-7 2000 | Cell Culture |
| Adaptation of total phenytoin reagent pack for measuring free phenytoin levels with the Abbott AxSYM immunoassay analyzer Jarzabek JI (REPRINT) ; Kampa IS Therapeutic drug monitoring 1999, v21, n1, p134-136 1999 | |
| Phenylalanine and tyrosine quantification by stable isotope dilution liquid chromatography-mass spectrometry from filter paper blood spots Tuchman, M. McCann, M. T. Clinical Chemistry (Washington, D. C.) VO- 45 IS- 4 PG- 571-573 Apr 1999 1999 | Sample Prep |
| Circadian rhythm of free melatonin in human plasma Kennaway DJ (REPRINT) ; Voultsios A JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM, 1998, V83, N3 MAR, P1013-1015 1998 | Clinical Lab, Protein Binding |
| Factors affecting the free plasma fraction of phenytoin in patients with epilepsy Jimenez MC; Duran JA (REPRINT) ; Abadin JA Clinical drug investigation, 1998, V15, P153-157 1998 | Clinical Lab |
| Differential analysis of blood urea using combined automated ultrafiltration and solid phase extraction in on-line series Shintani H.,Journal of Liquid Chromatography and Related Technologies (2205-2210) 1998 Journal of Liquid Chromatography and Related Technologies (2205-2210) 1998 1998 | |
| Effects of tryptophan depletion vs. catecholamine depletion in patients with seasonal affective disorder in remission with light therapy Neumeister, Alexander et al. Archives of general psychiatry, 1998 1998 | |
| Valproic acid-ketoconazole interaction in normal, hypoalbuminemic, and uremic sera: Lack of interaction in uremic serum caused by the presence of inhibitor Dasgupta Amitava(a); Luke Matthew; Therapeutic Drug Monitoring VO- 19 IS- 3 PG- 281-285 June, 1997 SN- 0163-4356 Therapeutic Drug Monitoring VO- 19 IS- 3 PG- 281-285 June, 1997 SN- 0163-4356 1997 |
FAQ
| Question | Answer |
|---|---|
| Can I use Centrifree with a swinging-bucket rotor? | Yes, but the filtration rate will be only 30-50% of that produced with a fixed-angle rotor at the same g-force. |
| What are the pore and retention characteristics of the YMT 30,000 molecular weight cut-off membrane for Centrifree micropartition device? | The actual pore size is not measured. The membrane is characterized by measuring its retention of known protein markers of specific molecular weights. The given cut-off is a nominal measurement. |
| Does Centrifree have a shelf life? | Yes, one year. |
| What is the maximum spinning speed of the Centrifree unit? | Device limits are measured in g-force, not rpm. The maximum recommended g-force is 2000 × g. |
| How much ultrafiltrate can I expect when using the Centrifree? | For volumes less than 0.5 ml: For a sample volume of 1.0 ml: In 10 minutes: I 1/2 the initial volume approximately 200 µl In 30 minutes: 2/3 to 3/4 the initial volume approximately 450 µl |
| What is the maximum process volume for the Centrifree device? | Maximum volume is 1.0 ml. |
| What molecular weight cut-off does the Centrifree come in? | The Centrifree unit is available in a 30,000 MW cut-off. |
| Can I reuse Centrifree? | No, Centrifree is intended for one-time use only. Multiple use risks membrane failure. |
| What is the minimum process volume for the Centrifree device? | Minimum volume is 0.15 ml. |
| What temperatures can Centrifree be spun at? | Spin devices at 4-50°C. Store at room temperature. |