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14-197M Casein Kinase 2 Protein, active, 250 µg

Active, recombinant full-length human Casein Kinase 2 protein, containing an α-subunit N-terminal 6His tag & a β-subunit N-terminal GST tag. For use in Kinase Assays.

Casein Kinase 2 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-197M
250 µg  Also available in 10 μg size (cat#14-197) and in bulk (cat# 14-197-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-197M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCasein Kinase 2 Protein, active, 250 µg
OverviewRecombinant full-length human Casein Kinase 2 protein, containing an &alpyha;-subunit N-terminal 6His tag and a β-subunit N-terminal GST tag.
References
Product Information
Presentation20mM MOPS, pH 7.4, 1mM EDTA, 0.1% (v/v) Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol.
Quality LevelMQ100
Applications
ApplicationActive, recombinant full-length human Casein Kinase 2 protein, containing an α-subunit N-terminal 6His tag & a β-subunit N-terminal GST tag. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryCasein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene.
Gene Symbol
  • CSNK2A1
  • CK2A1
  • CKII
Protein TargetCK2
Purification MethodSequential chromatography using heparin Sepharose™ and Mono Q anion exchange resin
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P68400 # Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
SIZE: 391 amino acids; 45144 Da
SUBUNIT: Tetramer composed of an alpha chain, an alpha' and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1.
SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain.
Molecular Weightheterotetramer of 49kDa α-subunit and 53kDa β-subunit (α2β2)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of the Casein Kinase 2 Substrate Peptide.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C.
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-197) and in bulk (cat# 14-197-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-197M 04053252672484

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-197 Casein Kinase 2 Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases