Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABT858 Anti-prelamin A Antibody, clone PL-1C7

Anti-prelamin A, clone PL-1C7, Cat. No. MABT858, is a mouse monoclonal antibody that detects Prelamin A and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Anti-prelamin A Antibody, clone PL-1C7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: LMNA, LMN1 UniProt Number: P02545
MABT858
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MELISA, FC, ICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT858
DescriptionAnti-prelamin A Antibody, clone PL-1C7
Alternate Names
  • LMNA
Background InformationPrelamin-A/C (UniProt: P02545; also known as 70 kDa lamin, Renal carcinoma antigen NY-REN-32) is encoded by the LMNA (also known as LMN1) gene (Gene ID: 4000) in human. Lamins are components of the nuclear lamina that provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A is initially synthesized as prelamin A that undergoes several modifications in the carboxyl terminal region that allow incorporation of prelamin A into the nuclear envelope and its subsequent processing into the mature lamin A. Cleavage of 15 residues (aa 647-662) by ZMPSTE24/FACE1 generates the final protein product. Unlike mature lamin A, prelamin A accumulates as discrete and localized foci at the nuclear periphery. Prelamin-A/C can accelerate smooth muscle cell senescence. It can act to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence. Mutations in LMNA gene are known to cause Emery-Dreifuss muscular dystrophy that is characterized by weakness and atrophy of muscle without involvement of the nervous system. Some mutations have also been linked to familial type of lipodystrophy characterized by the loss of subcutaneous adipose tissue in the lower parts of the body. (Ref.: Casasola, A., et al. (2016). Nucleus 7(1); 84-102).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-prelamin A, clone PL-1C7, Cat. No. MABT858, is a mouse monoclonal antibody that detects Prelamin A and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected prelamin A in U20S cells treated with farnesyl transferase inhibitor, Lonafarnib (3.2 uM for 24 h).

Flow Cytometry Analysis: A representative lot detected prelamin A in Flow Cytometry applications (Casasola, A., et. al. (2016). Nucleus. 7(1):84-102).

Western Blotting Analysis: A representative lot detected prelamin A in Western Blotting applications (Casasola, A., et. al. (2016). Nucleus. 7(1):84-102).

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected prelamin A in C2C12 cells with Farnesyl transferase inhibitor Lonafarnib (Courtesy of Fred Hutchinson Cancer Research Center, Seattle, Washington USA).

ELISA Analysis: A representative lot detected prelamin A in ELISA applications (Casasola, A., et. al. (2016). Nucleus. 7(1):84-102).

Immunocytochemistry Analysis: A representative lot detected prelamin A in Immunocytochemistry applications (Casasola, A., et. al. (2016). Nucleus. 7(1):84-102).
Biological Information
ImmunogenA lnear peptide corresponding to 12 amino acids from the C-terminal region of human Prelamin A.
EpitopeC-terminus
ClonePL-1C7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone PL-1C7 detects Prelamin A in human and murine cells. It specifically recognizes prelamin A at the intact ZMPSTE24 cleavage site.
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LMNA
  • LMN1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~74 kDa observed; 74.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected prelamin A in C2C12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT858 04054839396663

Documentation

Anti-prelamin A Antibody, clone PL-1C7 Certificates of Analysis

TitleLot Number
Anti-prelamin A, clone PL-1C7 - 3214390 3214390
Anti-prelamin A, clone PL-1C7 - 3254521 3254521
Anti-prelamin A, clone PL-1C7 - 3303095 3303095
Anti-prelamin A, clone PL-1C7 - 3490043 3490043
Anti-prelamin A, clone PL-1C7 - 3811704 3811704
Anti-prelamin A, clone PL-1C7 - 3903335 3903335
Anti-prelamin A, clone PL-1C7 - 3920871 3920871
Anti-prelamin A, clone PL-1C7 - 4069657 4069657
Anti-prelamin A, clone PL-1C7 - 4216896 4216896
Anti-prelamin A, clone PL-1C7 Monoclonal Antibody Q2959733
Show more