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07-052 Anti-phospho-Phospholamban (Ser16) Antibody

07-052
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, R, H, B, Ca, Po, RbWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-052
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Phospholamban (Ser16) Antibody
Alternate Names
  • cardiac phospholamban
Background InformationPhospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.
Product Information
FormatAffinity Purified
Control
  • Bovine cardiac preparation, treated with PKA.
PresentationPurified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Store at -20°C.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho-Phospholamban (Ser16) Antibody is validated for use in WB for the detection of phospho-Phospholamban (Ser16).
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) with an added C-terminal cysteine. The immunizing sequence is identical in mouse, rat, rabbit, pig and dog.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes phosphorylated phospho-lamban (Ser16), Mr 5-25 kDa. Additional non-specific bands may be detected, Mr 75 & 100 kDa, in some lysates.
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
  • Human
  • Bovine
  • Canine
  • Pig
  • Rabbit
Species Reactivity NoteBovine. Predicted to cross-react with human, mouse, rat, rabbit, pig and dog.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.
Gene Symbol
  • CMD1P
  • OTTHUMP00000017092
  • PLB
  • phospholamban
Modifications
  • Phosphorylation
Purification MethodImmunoaffinity Chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P26678 # Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum.
SIZE: 52 amino acids; 6109 Da
SUBUNIT: Homopentamer.
SUBCELLULAR LOCATION: Membrane.
TISSUE SPECIFICITY: Heart.
PTM: Phosphorylated in response to beta-adrenergic stimulation.
DISEASE:SwissProt: P26678 # Defects in PLN are the cause of dilated cardiomyopathy 1P (CMD1P) [MIM:609909]. Cardiomyopathy is a disorder characterized by cardiac dilation and reduced systolic function.
SIMILARITY: SwissProt: P26678 ## Belongs to the phospholamban family.
Molecular WeightVaries
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot on bovine cardiac preparation that was incubated with PKA.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10 µg PKA (Catalog # 14-114) for 10 minutes.
DO NOT BOIL CARDIAC PREPARATION.
Boiling may reduce the 25 kDa pentamer into tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
07-052 04053252469879