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16-305 Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor® 488

Detect phospho-Akt1/PKBα (Ser473) using this Anti-Akt1 Antibody, clone 11E6, Alexa Fluor 488 validated for use in FC, IC, IF & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: AKT1, RAC, PRKBA, MGC99656, RAC-ALPHA, RAC-PK-alpha, C-AKT, PKB, AKT UniProt Number: P31749
16-305
50 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ca, H, M, RFC, ICC, IF, WBMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number16-305
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor® 488
Alternate Names
  • PKB
  • PKD-alpha
  • AKT1
  • Protein Kinase B
References
Product Information
FormatAlexaFluor®488
HS Code3502 90 90
Quality LevelMQ100
Applications
ApplicationDetect phospho-Akt1/PKBα (Ser473) using this Anti-Akt1 Antibody, clone 11E6, Alexa Fluor 488 validated for use in FC, IC, IF & WB.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
  • Western Blotting
Biological Information
ImmunogenKLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.
Clone11E6
HostMouse
SpecificityRecognizes phosphorylatedAkt1/PKBα at Ser473. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.
IsotypeIgG1
Species Reactivity
  • Canine
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Gene Symbol
  • AKT1
  • RAC
  • PRKBA
  • MGC99656
  • RAC-ALPHA
  • RAC-PK-alpha
  • C-AKT
  • PKB
  • AKT
Modifications
  • Phosphorylation
Purification MethodPurified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight60 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunocytochemistry and flow cytometry.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Storage and Shipping Information
Storage Conditions1 year at 4°C from date of shipment
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
16-305 04053252510816

Documentation

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor® 488 SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor® 488 Certificates of Analysis

TitleLot Number
Anti-phospho-Akt1/PKB (Ser473), clone 11E6, Alexa Fluor#174; 488 conjugate (mouse monoclonal IgG1) - 2228057 2228057
Anti-phospho-Akt1/PKB (Ser473), clone 11E6, Alexa Fluor#174; 488 conjugate - 2045836 2045836
Anti-phospho-Akt1/PKB (Ser473), clone 11E6, Alexa Fluor#174; 488 conjugate - DAM1512132 DAM1512132
Anti-phospho-Akt1/PKB (Ser473), clone 11E6, Alexa Fluor#174; 488 conjugate - JBC1909546 JBC1909546
Anti-phospho-Akt1/PKB#225; (Ser473), clone 11E6, Alexa Fluor#174; 488 conjugate - DAM1606730 DAM1606730
Anti-phospho-Akt1/PKB#945; (Ser473), clone 11E6, Alexa Fluor#174; 488 conjugate (mouse monoclonal IgG1#954;) - 2145908 2145908
Anti-phospho-Akt1/PKBalpha; (Ser473), clone 11E6, Alexa Fluorreg; 488 conjugate - D6D026 D6D026
Anti-phospho-Akt1/PKBalpha; (Ser473), clone 11E6, Alexa Fluorreg; 488 conjugate - DAM1416542 DAM1416542
Anti-phospho-Akt1/PKBα (Ser473), clone 11E6, Alexa Fluor® 488 conjugate (mouse monoclonal IgG1K) 3006320
Anti-phospho-Akt1/PKBα (Ser473), clone 11E6, Alexa Fluor® 488 conjugate (mouse monoclonal IgG1κ) 2864975
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Alternative Format

Catalogue Number Description
05-669 Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6

Product Families

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Millipore’s phosphoAkt antibodies have been well validated and published. See below or a comprehensive list of our phosphoAkt products, based on the expertise of Upstate & Chemicon.
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies