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MABE1016 Anti-pan-ADP-ribose binding reagent

Anti-poly-ADP-ribose binding reagent is a reagent that selectively binds to both mono- and poly- ADP ribose for use in Western Blotting, Immunocytochemistry and Dot Blot.

Anti-pan-ADP-ribose binding reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: General Post-translation Modification
MABE1016
200 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormat
M, HWB, IP, DBE. coliPurified
Description
Catalogue NumberMABE1016
DescriptionAnti-pan-ADP-ribose binding reagent
Alternate Names
  • pan-ADP-ribose binding reagent
Background InformationCat. No.MABE1016, Anti-pan-ADP-ribose binding reagent, is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta™(DE3)pLysS strain of E. coli (Cat. No. 70956). Anti-pan-ADP-ribose binding reagent is useful for the affinity detection of both mono- and poly-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis, The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-pan-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified from E. coli by Ni-NTA agarose. Supplied in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10% Glycerol, 10 mM Imidazole, 1 mM PMSF, 1 mM β-Mercaptoethanol, 10% glycerol without preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-poly-ADP-ribose binding reagent is a reagent that selectively binds to both mono- and poly- ADP ribose for use in Western Blotting, Immunocytochemistry and Dot Blot.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Dot Blot
Application NotesDot Blot Specificity Analysis: This reagent detected mono(ADPR) on recombinant PARP3 protein, as well as mono(ADPR) and poly(ADPR) on recombinant PARP1 recombinant protein (Lee Kraus, University of Texas Southwestern Medical Center).

Immunoprecipitation Analysis: A representative lot of Anti-pan-ADP-ribose binding reagent immunoprecipitated ADP-ribosylated proteins from nuclear extract (Lee Kraus, University of Texas Southwestern).

Western Blotting Analysis: A representative lot detected auto-ADP-ribosylation activity of PARP1/2/3 and mutants in the presence of NAD+ or various NAD+ analogs (Gibson, B.A., et al. (2016). Science. 353(6294):45-50).

Western Blotting Analysis: A representative lot detected PARP1-catalyzed NELF-E ADP-ribosylation in cell-free enzymatic reactions as well as ADP-ribosylation of exogenously expressed FLAG-tagged NELF-E in HEK293T cells. PARP inhibitor PJ34 or P-TEFb/CDK9 inhibitor flavopiridol treatment decreased cellular NELF-E ADP-ribosylation level (Gibson, B.A., et al. (2016). Science. 353(6294):45-50).
Biological Information
ConcentrationPlease refer to lot specific datasheet.
HostE. coli
Specificitypoly(ADP-ribose) and mono(ADP-ribose)
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human. Predicted to react with all species.
Purification MethodNi-NTA agarose
Molecular WeightVariable depending on the target proteins and the extend of ADP-ribosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting on ADP-ribosylated PARP1 and PARP3 recombinant proteins.

Western Blotting Analysis: This reagent detected mono(ADPR) on recombinant PARP3 protein, as well as mono(ADPR) and poly(ADPR) on recombinant PARP1 protein (Lee Kraus, University of Texas Southwestern Medical Center).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -80°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1016 04055977168266

Documentation

Anti-pan-ADP-ribose binding reagent SDS

Title

Safety Data Sheet (SDS) 

Anti-pan-ADP-ribose binding reagent Certificates of Analysis

TitleLot Number
Anti-pan-ADP-ribose binding reagent 3074935
Anti-pan-ADP-ribose binding reagent 3772221
Anti-pan-ADP-ribose binding reagent 2918927
Anti-pan-ADP-ribose binding reagent 3126639
Anti-pan-ADP-ribose binding reagent 3013396
Anti-pan-ADP-ribose binding reagent 3043706
Anti-pan-ADP-ribose binding reagent 3153240
Anti-pan-ADP-ribose binding reagent 2958005
Anti-pan-ADP-ribose binding reagent 2836182
Anti-pan-ADP-ribose binding reagent 2901597
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Technical Info

Title
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White Paper: Further considerations of antibody validation and usage.