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OP33 Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)

This Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-5) (Wild type).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP33
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, Pm, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP33-100UG Plastic ampoule 100 μg
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OP33-20UG Plastic ampoule 20 μg
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Description
OverviewRecognizes the ~53 kDa wild-type p53 protein in its native conformation in Hs27 cells and breast carcinoma tissue. Does not recognize mutant or denatured p53 protein.
Catalogue NumberOP33
Brand Family Calbiochem®
References
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Ball, R.K., et al. 1984. EMBO J. 3, 1485.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive controlHs27 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.
Key Applications Frozen Sections
Immunofluorescence
Immunoprecipitation
Not Western Blot
Paraffin Sections
Application NotesFrozen Sections (see applications references)
Immunoblotting (not recommended)
Immunofluorescence (1-20 μg/ml)
Immunoprecipitation (1 μg per sample)
Paraffin Sections (5 μg/ml, heat pre-treatment required)
Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenvLM mouse tumor cells
ImmunogenMouse
ClonePAb1620
HostMouse
IsotypeIgG2a
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Primate
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
OP33-100UG 04055977208443
OP33-20UG 07790788053901