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OP15 Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)

OP15
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP15-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells.
Catalogue NumberOP15
Brand Family Calbiochem®
SynonymsAnti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2
Application Data
Detection of human c-ErbB2/c-Neu by immunoblotting. Sample: Whole cell lysate from HEK293 cells. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5) (Cat. No. OP15) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesMamcs, J.R., et al. 1994. Ann. Surg. 219, 332.
Tetu, B., et al. 1994. Cancer 73, 2359.
DiFiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Bargmann, C.I., et al. 1986. Nature 319, 226.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlHepG2 cells or normal skin
Positive controlSK-BR-3 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections Meissner, K., et al. 1990. Am. J. Pathol. 137, 1305. Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239. Original Clone Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239. Immunohistochemistry, Immunoblotting Zhau, H.E., et al. 1990. Mol. Carcinogen. 3, 254. Immunoprecipitation Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (2.5 µg/ml)
Immunoblotting (2-3 µg/ml, see application references)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/sample, see application references)
Paraffin Sections (2.5 µg/ml, pressure cooker, heat or trypsin pre-treatment required, see application references)
Application CommentsFor paraffin sections, we recommend pre-treating with a pressure cooker, but trypsin and heat will also work well. Including 0.05% saponin during staining of paraffin sections may help reduce background. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribriform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu
ImmunogenHuman
Clone3B5
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
OP15-100UG 04055977224658