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05-511-AF488 Anti-TCF-4 Antibody, clone 6H5-3 Antibody, AlexaFluor® 488 Conjugate

This Anti-TCF-4 Antibody, clone 6H5-3 Antibody, AlexaFluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of TCF-4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: TCF7L2, TCF4 UniProt Number: Q9NQB0
05-511-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number05-511-AF488
DescriptionAnti-TCF-4 Antibody, clone 6H5-3 Antibody, AlexaFluor® 488 Conjugate
Alternate Names
  • Transcription factor 7-like 2
  • HMG box transcription factor 4
  • T-cell-specific transcription factor 4
  • T-cell factor 4
  • TCF-4
  • hTCF-4
  • TCF-4
Background InformationTranscription factor 7-like 2 (UniProt Q9NQB0; also known as HMG box transcription factor 4, hTCF-4, T-cell factor 4, T-cell-specific transcription factor 4, TCF-4) is encoded by the TCF7L2 (also known as TCF4) gene (Gene ID 6934) in human.
TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-TCF-4 Antibody, clone 6H5-3 Antibody, AlexaFluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of TCF-4.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TCF-4 in HeLa, HUVEC, and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-511) is shown to be suitable also for Western blotting, Electrophoretic mobility shift assay (EMSA), immunocytochemistry, immunohistochemistry, and immunoprecipitation applications.
Biological Information
ImmunogenAmino acids 31-331 of human TCF-4.
Epitopea.a. 31-331
Clone6H5-3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2a
Species Reactivity
  • Mouse
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TCF7L2
  • TCF4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~66 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected TCF-4 in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-511-AF488 04055977298000