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DR1057 Anti-SQSTM1 Mouse mAb (2C11)

DR1057
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DR1057-100UG 100 μg
Description
OverviewRecognizes the SQSTM1 protein HeLa cells.
Catalogue NumberDR1057
Brand Family Calbiochem®
SynonymsAnti-Sequestosome 1
Application Data
Detection of human SQSTM1 by immunoblotting. Sample: Full-length, recombinant human protein. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human SQSTM1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SQSTM1 Mouse mAb (2C11) (Cat. No. DR1057) (10 µg/ml). Detection: fluorescence.
References
ReferencesLee, H,K., et al. 2010. Immunity. 32, 227.
Bjorkoy, G., et al. 2009. Methods Enzymol. 452, 181.
Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet. 18 4868.
Gal, J., et al. 2009. J. Neurochem. 111 1062.
Mardakheh, F,K., et al. 2009.J. Cell Biol. 187, 265.
Jung, H,S., et al. 2008. Cell Metab. 8, 318.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein
ImmunogenNA
Clone2C11
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
DR1057-100UG 04055977226010