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ABS1848 Anti-Rho (-A, -B, -C) Antibody

Anti-Rho (-A, -B, -C) Antibody is an antibody against Rho (-A, -B, -C) for use in Western Blotting.

Anti-Rho (-A, -B, -C) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: G-proteins Gene Symbol: RHOA, ARH12, ARHA, RHO12, RHOB, ARH6, ARHB, RHOC, ARH9, ARHC UniProt Number: P61586, P62745, P08134
ABS1848
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1848
DescriptionAnti-Rho (-A, -B, -C) Antibody
Alternate Names
  • Transforming protein RhoA
  • h12
  • Rho-related GTP-binding protein RhoB
  • h6
  • Rho-related GTP-binding protein RhoC
  • h9
Background InformationRac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Rho (-A, -B, -C) Antibody is an antibody against Rho (-A, -B, -C) for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated synthetic peptide (SYPDTDVILMCFSIDSPDSLEN-KK) corresponding to amino acids 73-94 of human RhoA & RhoB, with two C-terminal lysines added to increase peptide solubility corresponding to human Rho (-A, -B, -C).
EpitopeAmino acids 73-94.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody is raised against an internal sequence highly conserved among RhoA (100%), RhoB (95%), and RhoC (100%).
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rat, Chicken, Mouse, Nematode, Zebrafish, Xenopus based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RHOA
  • ARH12
  • ARHA
  • RHO12
  • RHOB
  • ARH6
  • ARHB
  • RHOC
  • ARH9
  • ARHC
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~22 kDa observed. 21.44 kDa (human RhoA), 21.78 kDa (human RhoB), and 21.68 kDa (human RhoC) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected RhoA family GTPase in 10 µg of HL-60 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1848 04055977349429