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OP40 Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10)

This Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of Pan-Ras (Ab-3).

Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP40
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP40-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~21 kDa H-, K-, and N-Ras proteins in SW480 and Y1 cells and breast carcinoma tissue.
Catalogue NumberOP40
Brand Family Calbiochem®
Application Data
Detection of human Ras by immunoblotting: Sample: Whole cell lysate (56 μg) from MCF-7 cells. Primary antibody: Anti-Pan-Ras (Ab-3) Mouse mAb (RAS10) (Cat. No. OP40) (0.3 μg/ml). Detection: chemiluminescence.
References
ReferencesRodenhuis, S. et al., 1992. Cancer Res. (Suppl) 52, 2665s.
Sidransky, D., et al. 1992. Science 256, 102.
Hamer, P.J., et al. 1990. Hybridoma 9, 573.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M. E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Positive controlSW480 or Y1 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone, Immunoblotting, Immunoprecipitation Hamer, P.J., et al. 1990. Hybridoma 9, 573.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesFlow Cytometry (2.5 µg/ml)
Frozen Sections (2.5 µg/ml)
Immunoblotting (0.1-3 µg/ml, chemiluminescent and colorimetric, see application references)
Immunofluorescence (2.5 µg/ml, see application references)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (2.5 µg/ml, saponin pre-treatment required)
Application CommentsThe level of expression of p21ras is variable in different tissues. For this reason we recommend a concentration step prior to immunoblot analysis to obtain optimal results. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human p21 Ras
ImmunogenHuman
CloneRAS 10
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP40-100UG 07790788054458

Documentation

Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) SDS

Title

Safety Data Sheet (SDS) 

Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) Certificates of Analysis

TitleLot Number
OP40

References

Reference overview
Rodenhuis, S. et al., 1992. Cancer Res. (Suppl) 52, 2665s.
Sidransky, D., et al. 1992. Science 256, 102.
Hamer, P.J., et al. 1990. Hybridoma 9, 573.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M. E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.

Citations

Title
  • Marie Dance, et al. (2006) The adaptor protein gab1 couples the stimulation of vascular endothelial growth factor receptor-2 to the activation of phosphoinositide 3-kinase. Journal of Biological Chemistry 281, 23285-23295.
  • Clair C. Sharpe, et al. (1999) Evidence of a Role for Ki-RAS in the Stimulated Proliferation of Renal Fibroblasts. Journal of the American Society of Nephrology 10, 1186-1192.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision26-September-2007 JSW
    ApplicationFlow Cytometry (2.5 µg/ml)
    Frozen Sections (2.5 µg/ml)
    Immunoblotting (0.1-3 µg/ml, chemiluminescent and colorimetric, see application references)
    Immunofluorescence (2.5 µg/ml, see application references)
    Immunoprecipitation (1 µg/sample)
    Paraffin Sections (2.5 µg/ml, saponin pre-treatment required)
    Application Data
    Detection of human Ras by immunoblotting: Sample: Whole cell lysate (56 μg) from MCF-7 cells. Primary antibody: Anti-Pan-Ras (Ab-3) Mouse mAb (RAS10) (Cat. No. OP40) (0.3 μg/ml). Detection: chemiluminescence.
    DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 myeloma cells (see application references). Recognizes the ~21 kDa Ras protein.
    BackgroundThe human ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging.
    HostMouse
    Immunogen speciesHuman
    Immunogenrecombinant human p21 Ras
    CloneRAS 10
    IsotypeIgG2a
    Specieshuman, mouse, rat
    Positive controlSW480 or Y1 cells or breast carcinoma tissue
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsThe level of expression of p21ras is variable in different tissues. For this reason we recommend a concentration step prior to immunoblot analysis to obtain optimal results. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesRodenhuis, S. et al., 1992. Cancer Res. (Suppl) 52, 2665s.
    Sidransky, D., et al. 1992. Science 256, 102.
    Hamer, P.J., et al. 1990. Hybridoma 9, 573.
    Bos, J.L. 1989. Cancer Res. 49, 4682.
    Furth, M. E., et al. 1987. Oncogene 1, 47.
    Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
    Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
    Taparowsky, E., et al. 1983. Cell 34, 581.
    Ellis, R.W., et al. 1981. Nature 292, 506.
    Shih, T.Y., et al. 1980. Nature 287, 686.
    Citation
  • Marie Dance, et al. (2006) The adaptor protein gab1 couples the stimulation of vascular endothelial growth factor receptor-2 to the activation of phosphoinositide 3-kinase. Journal of Biological Chemistry 281, 23285-23295.
  • Clair C. Sharpe, et al. (1999) Evidence of a Role for Ki-RAS in the Stimulated Proliferation of Renal Fibroblasts. Journal of the American Society of Nephrology 10, 1186-1192.
    		•
    Application referencesOriginal Clone, Immunoblotting, Immunoprecipitation Hamer, P.J., et al. 1990. Hybridoma 9, 573.