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MAB1393-I Anti-PECAM-1 (CD31) Antibody, clone TLD-3A12

This mouse monoclonal Anti-PECAM-1 (CD31), clone TLD-3A12, Cat. No. MAB1393-I is tested for use in ELISA, Flow Cytometry, Functional Studies, Immunohistochemistry (Paraffin) and Immunohistochemistry, Immunoprecipitation, and Western Blotting, for the detection of CD31/PECAM-1.

Anti-PECAM-1 (CD31) Antibody, clone TLD-3A12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Pecam1, Pecam UniProt Number: Q3SWT0
MAB1393-I
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RIHC, FUNC, WB, FC, ELISA, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1393-I
DescriptionAnti-PECAM-1 (CD31) Antibody, clone TLD-3A12
Alternate Names
  • Platelet endothelial cell adhesion molecule
  • CD31
Background InformationPlatelet endothelial cell adhesion molecule (UniProt: Q3SWT0; PECAM-1, CD31) is encoded by the Pecam1 (also known as Pecam) gene (Gene ID: 29583) in rat.PECAM-1 is a single-pass type I membrane protein that contains 6 Ig-like C2-type (immunoglobulin-like) domains. PECAM1 is differentially glycosylated involving both N-linked and O-linked glycosylation sites. It is a cell adhesion molecule that is required for leukocyte trans-endothelial migration (TEM) under most inflammatory conditions. PECAM1 plays a vital role in signaling processes involved in angiogenesis, platelet function, thrombosis, mechanosensing of endothelial cell response to fluid shear stress, and regulation of multiple stages of leukocyte migration through venular walls. PECAM1 is expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. PECAM1 prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes. During apoptosis, the inside-out signaling of PECAM1 is disabled that allows the apoptotic cell to accept phagocyte interaction. PECAM1 is phosphorylated on serine and tyrosine residues following cellular activation. In endothelial cells Fyn is shown to mediate mechanical-force induced tyrosine phosphorylation. In response to Fc epsilon RI (FCER1 activation), PECAM1 is phosphorylated on tyrosine residues by FER and FES.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-PECAM-1 (CD31), clone TLD-3A12, Cat. No. MAB1393-I is tested for use in ELISA, Flow Cytometry, Functional Studies, Immunohistochemistry (Paraffin) and Immunohistochemistry, Immunoprecipitation, and Western Blotting, for the detection of CD31/PECAM-1.
Key Applications
  • Immunohistochemistry
  • Affects Function
  • Western Blotting
  • Flow Cytometry
  • ELISA
  • Immunohistochemistry (Paraffin)
Application NotesImmunoprecipitation Analysis: A representative lot detected PECAM-1 (CD31) in Immunoprecipitation applications (Williams, K.C., et. al. (1996). J Neurosci Res. 45(6):747-57).

Flow Cytometry Analysis: 1 µg from a representative lot detected PECAM-1 (CD31) in one million rat splenocytes.

Western Blotting Analysis: A representative lot detected PECAM-1 (CD31) in Western Blotting applications (Male, D., et. al. (1995). Immunology. 84(3):453-60).

ELISA Analysis: A representative lot detected PECAM-1 (CD31) in ELISA applications (Male, D., et. al. (1995). Immunology. 84(3):453-60; Williams, K.C., et. al. (1996). J Neurosci Res. 45(6):747-57).

Affects Function: A representative lot of PECAM-1 (CD31) Affected Function (Williams, K.C., et. al. (1996). J Neurosci Res. 45(6):747-57).

Immunohistochemistry Analysis: A representative lot detected PECAM-1 (CD31) in Immunohistochemistry applications (Williams, K.C., et. al. (1996). J Neurosci Res. 45(6):747-57).
Biological Information
ImmunogenLewis rat derived activated microglial cells.
CloneTLD-3A12
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone TLD-3A12 recognizes rat and human PECAM-1.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Pecam1
  • Pecam
Purification MethodProtein G purified
UniProt Number
Molecular Weight76.19 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human tonsil.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected PECAM-1 (CD31) in human tonsil tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB1393-I 04054839199325

Documentation

Anti-PECAM-1 (CD31) Antibody, clone TLD-3A12 SDS

Title

Safety Data Sheet (SDS) 

Anti-PECAM-1 (CD31) Antibody, clone TLD-3A12 Certificates of Analysis

TitleLot Number
Anti-PECAM-1 (CD31), clone TLD-3A12 - 3308162 3308162
Anti-PECAM-1 (CD31), clone TLD-3A12 - 3526285 3526285
Anti-PECAM-1 (CD31), clone TLD-3A12 - 4045766 4045766
Anti-PECAM-1 (CD31), clone TLD-3A12 - 4109622 4109622
Anti-PECAM-1 (CD31), clone TLD-3A12 Monoclonal Antibody 3036819
Anti-PECAM-1 (CD31), clone TLD-3A12 Monoclonal Antibody 3096939
Anti-PECAM-1 (CD31), clone TLD-3A12, Monoclonal Antibody Q2841548