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CBL468 Anti-PECAM-1 Antibody, domains 3-6 of human PECAM-1, clone HC1/6

CBL468
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IHC, IH(P), IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL468
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-PECAM-1 Antibody, domains 3-6 of human PECAM-1, clone HC1/6
Alternate Names
  • CD31
Product Information
FormatPurified
Control
  • Activated microglial cells
PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationAnti-PECAM-1 Antibody, domains 3-6 of human PECAM-1, clone HC1/6 is an antibody against PECAM-1 for use in FC, IH, IH(P) & IP.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
Application NotesStudies of the role of CD31 in leucocyte migration into inflammatory sites and in the process of angiogenesis

This antibody will stain frozen tissue sections

Suitable for immunoprecipitation studies

Suitable for flow cytometry either indirectly or directly when labelled with FITC or R-PE

Suitable for use on formalin fixed paraffin embedded sections after protease pre-treatment.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPMA - treated U937 cells
Epitopedomains 3-6 of human PECAM-1
CloneHC1/6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with a 130-140 kDa transmembranous glycoprotein that is expressed widely amongst cells of the vascular compartment. CD31 is expressed on platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney as well as in skin biopsies from conditions such as sarcoidosis and lepromatous leprosy.
FUSION PARTNER: P3-x63Ag8.653 myeloma cell line
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PECAM1
  • EndoCAM
  • GPIIA
  • PECAM-1
  • CD31
  • CD31/EndoCAM
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.
SIZE: 738 amino acids; 82536 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.
PTM: Phosphorylated on Ser and Tyr residues after cellular activation.
SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
CBL468 04053252331015