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MABC969 Anti-PD-L2 Antibody, clone 24F.10C12

This Anti-PD-L2 Antibody, clone 24F.10C12 is validated for use in Western Blotting, Flow Cytometry, Neutralizing, Immunohistochemistry for the detection of PD-L2.

Anti-PD-L2 Antibody, clone 24F.10C12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: PDCD1LG2, B7DC, CD273, PDCD1L2, PDL2 UniProt Number: Q9BQ51
MABC969
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FC, NEUT, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC969
DescriptionAnti-PD-L2 Antibody, clone 24F.10C12
Alternate Names
  • Programmed cell death 1 ligand 2
  • B7-DC
  • B7 dendritic cell molecule
  • B7-H2, Butyrophilin B7-DC
  • CD273
  • PD-1 ligand 2
  • PD-L2
  • PDCD1 ligand 2
  • Programmed death ligand 2
Background InformationProgrammed cell death 1 ligand 2 (UniProt Q9BQ51; also known as B7-DC, B7 dendritic cell molecule, B7-H2, Butyrophilin B7-DC, CD273, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2) is encoded by the PDCD1LG2 (also known as B7DC, CD273, PDCD1L2, PDL2) gene (Gene ID 80380) in human. PD-1 and PD-1 ligands 1&2 (PD-L1 and PD-L2) are B7:CD28 family members that regulate T cell activation and peripheral tolerance. When engaged together with the TCR, the interaction of PD-1 with its ligands delivers an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and nonhematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Under some conditions, blockade of this pathway is able to restore many T cell functions. PD-L2 is initially produced with signal peptide (a.a. 1-19) sequence, the removal of which yields the mature protein with a large extracellular (a.a. 20-220) region that contains an Ig-like V-type domain (a.a. 21-118) and an Ig-like C2-type domain (a.a. 122-203), followed by a transmembrane domain (a.a. 221-241) and a cytoplasmic tail (a.a. 242-273).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-PD-L2 Antibody, clone 24F.10C12 is validated for use in Western Blotting, Flow Cytometry, Neutralizing, Immunohistochemistry for the detection of PD-L2.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • Neutralizing
  • Immunohistochemistry
Application NotesFlow Cytometry Analysis: 0.1 µg from a representative lot detected PD-L2-positive human PBMCs.
Flow Cytometry Analysis: A representative lot immunostained the surface of 300.19 murine pre-B lymphoma cells transfected with human PD-L2, but not the untransfected cells or cells transfected with human PD-L1 (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Flow Cytometry Analysis: A representative lot was conjugated with FITC and detected a small induction of surface PD-L2 expression on human PBMCs in culture upon IFN-gamma stimulation (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Flow Cytometry Analysis: A representative lot was conjugated with FITC and employed to detect surface PD-L2 expression on PBMC-derived dendric cells (DCs). An upregulated PD-L2 expression was seen among mature DCs (mDC) than immature DCs (iDC), the levels of PD-L2 were reduced upon pretreatment of mDC with IL-10 (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Flow Cytometry Analysis: A representative lot was conjugated with FITC and detected surface PD-L2 expression on tonsil-derived human follicular DCs (FDCs) (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Neutralizing Analysis: A representative lot competed against human PD-1 extracellular domain Ig fusion for the binding of exogenously expressed human PD-L2 on the surface of 300.19 murine pre-B lymphoma cells (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Neutralizing Analysis: DP-L2 blocking on the surface of PBMC-derived dendritic cells (DCs) by clone 24F.10C12 Fab fragment increased CD4+ T cell proliferation and cytokine production in allogenic cultures with immature DCs (iDC), mature DCs (mDC), and IL-10-treated mDCs. Dual blockage of both DP-L1 (by clone 29E.2A3 Fab fragment) and DP-L2 synergized the enhancing effect (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Immunohistsochemistry Analysis: A representative lot detected PD-L2 expression pattern in frozen human tonsil, placenta, fetal thymus and cardiac tissue sections (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266).
Immunocytsochemistry Analysis: A representative lot detected an upregulated PD-L2 immunoreactivity in human CD14+ monocyte-derived macrophages (MDMs) following HIV-BaL infection, but not LPS stimulation by fluorescent immunocytochemistry using paraformaldehyde-fixed MDMs (Rodríguez-García, M., et al. (2011). J. Leukoc. Biol. 89(4) 507–515).
Biological Information
ImmunogenRecombinant human PD-L2.
EpitopeExtracellular domain.
Clone24F.10C12
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 24F.10C12 immunostained the surface of 300.19 murine pre-B lymphoma cells transfected with human PD-L2, but not the untransfected cells or cells transfected with human PD-L1 (Brown, J.A., et al. (2003). J. Immunol. 170(3):1257-1266). Reactivity toward spliced isoform 2 (PD-L2II) and isoform 3 (PD-L2III) has not been determined.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PDCD1LG2
  • B7DC
  • CD273
  • PDCD1L2
  • PDL2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~30 kDa observed. 28.85 kDa (isoform 1; PD-L2I), 18.76 kDa (isoform 2; PD-L2II), 18.64 kDa (isoform 3; PD-L2III) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human thymus tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PD-L2 in 10 µg of human thymus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC969 04055977331226

Documentation

Anti-PD-L2 Antibody, clone 24F.10C12 SDS

Title

Safety Data Sheet (SDS) 

Anti-PD-L2 Antibody, clone 24F.10C12 Certificates of Analysis

TitleLot Number
Anti-PD-L2, clone 24F.10C12 - 3188628 3188628
Anti-PD-L2, clone 24F.10C12 - 3419585 3419585
Anti-PD-L2, clone 24F.10C12 - 3731607 3731607
Anti-PD-L2, clone 24F.10C12 - 3781055 3781055
Anti-PD-L2, clone 24F.10C12 - 3859713 3859713
Anti-PD-L2, clone 24F.10C12 - 4093967 4093967
Anti-PD-L2, clone 24F.10C12 -Q2652394 Q2652394
Anti-PD-L2, clone 24F.10C12 Monoclonal Antibody 3096931
Anti-PD-L2, clone 24F.10C12 Monoclonal Antibody 3033468