Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

05-233 Anti-Nitrotyrosine Antibody, clone 1A6

05-233
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtICC, IHC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-233
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Nitrotyrosine Antibody, clone 1A6
Background InformationNitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others
Product Information
FormatPurified
Control
  • Immunohistochemistry: Human Alzheimer disease brain sections (Methacarn fixed), mouse liver
    Immunoblotting: Nitrated-BSA
    Indirect ELISA: nitrated-BSA
    Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine
PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-Nitrotyrosine Antibody, clone 1A6 detects level of Nitrotyrosine & has been published & validated for use in IC, IH, IP & WB.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Biological Information
ImmunogenNitrated KLH
Clone1A6
HostMouse
SpecificityRecognizes nitrated protein.
IsotypeIgG2bκ
Species Reactivity
  • Vertebrates
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular WeightPredicted MW for nitrated-BSA: 66 kDa
Product Usage Statements
Quality Assuranceroutinely evaluated on peroxynitrite-treated A431 cells; no reaction was noted with non-nitrated proteins
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
05-233 04053252622977