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MABE78 Anti-MiTF Antibody, clone 3D1.2

Anti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC.

Anti-MiTF Antibody, clone 3D1.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Transcription Factors Gene Symbol: MITF, BHLHE32 UniProt Number: O75030
MABE78
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE78
ReplacesMAB3747
DescriptionAnti-MiTF Antibody, clone 3D1.2
Alternate Names
  • Microphthalmia-associated transcription factor
  • Class E basic helix-loop-helix protein 32
  • bHLHe32
Background InformationMicrophthalmia-associated transcription factor (MiTF) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, and development of mast cells and bone. Mutations in MiTF cause auditory pigmentary syndromes in humans, such as Waardenburg syndrome type II and type IIa, and Tietz syndrome. There are two known isoforms of MiTF, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, whereas the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
References
Product Information
FormatPurified
Control
  • Mouse brain tissue lysate
PresentationPurified mouse monoclonal IgMκ in buffer containing 0.01M PBS, 0.1% Sodium Azide, pH 7.1.
Quality LevelMQ100
Applications
ApplicationAnti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:100 dilution of this antibody detected MiTF in human melanoma tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human MiTF.
Clone3D1.2
HostMouse
IsotypeIgMκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MITF
  • BHLHE32
UniProt Number
Molecular Weight~52 kDa observed. Multiple isoforms between 46-56 kDa are known to exist.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE78 04053252698842

Documentation

Anti-MiTF Antibody, clone 3D1.2 SDS

Title

Safety Data Sheet (SDS) 

Anti-MiTF Antibody, clone 3D1.2 Certificates of Analysis

TitleLot Number
Anti-MiTF, clone 3D1.2 - 2562843 2562843
Anti-MiTF, clone 3D1.2 - 3270773 3270773
Anti-MiTF, clone 3D1.2 - 3734543 3734543
Anti-MiTF, clone 3D1.2 - Q2060583 Q2060583
Anti-MiTF, clone 3D1.2 -2650908 2650908
Anti-MiTF, clone 3D1.2 -2712568 2712568
Anti-MiTF, clone 3D1.2 -2750089 2750089
Anti-MiTF, clone 3D1.2 -2793645 2793645
Anti-MiTF, clone 3D1.2 Monoclonal Antibody 3050686
Anti-MiTF, clone 3D1.2 Monoclonal Antibody 3499348