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ABN1464 Anti-GRP-78

Anti-GRP-78, Cat. No. ABN1464, is a highly specific rabbit polyclonal antibody that targets 78 kDa glucose-regulated protein and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-GRP-78 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: HSPA5, GRP78 UniProt Number: P11021
ABN1464
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), WB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN1464
DescriptionAnti-GRP-78
Alternate Names
  • 78 kDa glucose-regulated protein
  • GRP-78
  • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
  • Heat shock 70 kDa protein 5
  • Immunoglobulin heavy chain-binding protein
  • BiP
Background Information78 kDa glucose-regulated protein (UniProt: P11021; also known as GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP) is encoded by the HSPA5 (also known as GRP78) gene (Gene ID: 3309) in human. GRP78 a member of the HSP family of molecular chaperones that is required for stress-induced autophagy and for endoplasmic reticulum (ER) integrity. GRP78 is involved in multiple processes, including translocation of the newly formed proteins across the ER membrane, folding and assembly of proteins, and targeting misfolded proteins for ER-associated degradation. It plays a critical role in regulating unfolded protein response (UPR). Following ER stress, GRP78 is released from ER transmembrane signal transducers, such as PKR-like ER kinase (PERK), which leads to the activation of UPR signaling pathways. Once GRP78 is dissociated, PERK is allowed to dimerize and is autophosphorylated and activated. Activated PERK phosphorylates elF2alpha that results in the reduction of protein synthesis. (Ref.:Wang, M., et al. (2009). Antioxid Redox Signal. 11(9): 2307 2316).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GRP-78, Cat. No. ABN1464, is a highly specific rabbit polyclonal antibody that targets 78 kDa glucose-regulated protein and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 0.2 µg/mL from a representative lot detected GRP-78 in 10 µg of A431 cell lysates.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GRP-78 in human cerebellum, human pancreas, human small intestine, and human tonsil tissues.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GRP-78 in NIH/3T3 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human GRP-78.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects GRP-78 protein in multiple mammalian species. It targets an epitope within 17 amino acids from the C-terminal region.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Goat, Ground Squirrel, Hamster, Mouse, and Primates based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HSPA5
  • GRP78
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~78 kDa observed; 72.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysates.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected GRP-78 in 10 µg of A431 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1464 04054839199332