Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABE2696 Anti-GADD45 alpha Antibody

This Anti-GADD45 alpha Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GADD45 alpha.

Anti-GADD45 alpha Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: GADD45A, DDIT1, GADD45 UniProt Number: P24522
ABE2696
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE2696
Replaces07-1230
DescriptionAnti-GADD45 alpha Antibody
Alternate Names
  • Growth arrest and DNA damage-inducible protein GADD45 alpha
  • DDIT-1
  • DNA damage-inducible transcript 1 protein
Background InformationGrowth arrest and DNA damage-inducible protein GADD45 alpha (UniProt P24522; also known as DDIT-1, DNA damage-inducible transcript 1 protein) is encoded by the GADD45A (also known as DDIT1, GADD45) gene (Gene ID 1647) in human. GADD45A, GADD45B, and GADD45G constitute a family of sensors that can mediate rapid responses to a variety of stressors in mammals. GADD45 proteins are involved in cellular DNA repair, cell cycle, cell survival, apoptosis, senescence, and DNA demethylation under various stress conditions. The interaction of GADD45 with PCNA regulates DNA replication and repair by stimulating nucleotide excision repair. GADD45 can induce cell cycle arrest through direct interaction with cell cycle-related protein CDK1 and p21. Direct phosphorylation of MEKK4 by GADD45 leads to activation of JNK and p38 kinase, resulting in cellular apoptosis and senescence, respectively. All GADD45 proteins are able to interact with each other and form homo- or hetero-dimers, which are crucial for GADD45 functions. GADD45 proteins are ubiquitously expressed, but can vary in their tissue expression patterns among the three members. Gadd45a deletion in mice causes genomic instability, resulting in defective mitosis and cytokinesis, as wll as increased radiation carcinogenesis. In addition to the reported antitumor activity via the induction of cellular apoptosis or senescence in cancer cells, GADD45A has also been shown to play a role in facilitating tumorigenesis in certain cancers.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-GADD45 alpha Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GADD45 alpha.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected GADD45 alpha dimer in 10 µg of HEK293 cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GADD45 alpha in human colon cancer tissue section.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminal sequence of human GADD45 alpha.
EpitopeN-terminus.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects GADD45 alpha. Immunogen sequence is not conserved in GADD45 beta.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Bovine, Porcine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GADD45A
  • DDIT1
  • GADD45
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~18/36 kDa (monomer/dimer) observed. 18.34 kDa (monomer) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected GADD45 alpha in 10 µg of mouse kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE2696 04055977355369