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AB3534P Anti-Epithelial Sodium Channel-γ Antibody

AB3534P
50 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RELISA, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3534P
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Epithelial Sodium Channel-γ Antibody
Alternate Names
  • ENaC-gamma
Product Information
FormatAffinity Purified
PresentationAffinity purified immunoglobulin. Liquid in PBS, containing 0.1% BSA.
Quality LevelMQ100
Applications
ApplicationDetect Epithelial Sodium Channel-γ using this Anti-Epithelial Sodium Channel-γ Antibody validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern blot: 1-10 μg/mL using ECL. 83-90kDa bands; Masilamani et al 1999

ELISA: 1:10,000-1:100,000 using 1 mg/mL control peptide coated on well.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenA 14 amino acid peptide sequence near the N-terminus of rat ENaC gamma (Linguela et al. 1993; Canessa et al.

1993; Snyder et al. 1994; Waldmann et al. 1995; Kreutz et al. 1997; Voilley et al.

1994; McDonald et al. 1994; Ludwig et al. 1998; Garty & Palmer 1997).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes rat Epithelial Sodium Channel gamma (ENaC gamma). The immunogen shows no significant sequence homology with other ENaC subunits.

SPECIES REACTIVITIES: The immunogen sequence is 100% conserved in mouse, 92% in human, 80% in rabbit. Reactivity with other species has not been confirmed.
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SCNN1G
  • Gamma-ENaC
  • ENaCg
  • ENaCgamma
  • PHA1
  • SCNEG
  • Gamma-NaCH
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P51170 # Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
SIZE: 649 amino acids; 74270 Da
SUBUNIT: Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2.
SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note=Apical membrane of epithelial cells.
PTM: Phosphorylated on serine and threonine residues (By similarity). & Ubiquitinated; this targets individual subunits for endocytosis and proteasome-mediated degradation (By similarity).
DISEASE: SwissProt: P51170 # Defects in SCNN1G are a cause of Liddle syndrome [MIM:177200]. It is an autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel.
SIMILARITY: SwissProt: P51170 ## Belongs to the amiloride-sensitive sodium channel family.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µg
Global Trade Item Number
Catalogue Number GTIN
AB3534P 04053252333309