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ABD24 Anti-Dermo-1 Antibody

Anti-Dermo-1 Antibody is an antibody against Dermo-1 for use in WB, IH(P).

Anti-Dermo-1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Transcription Factors Gene Symbol: TWIST2, BHLHA39, DERMO1 UniProt Number: Q8WVJ9
ABD24
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, ChWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABD24
DescriptionAnti-Dermo-1 Antibody
Alternate Names
  • Twist-related protein 2
  • Class A basic helix-loop-helix protein 39
  • bHLHa39
  • Dermis-expressed protein 1
  • Dermo-1
Background InformationDermo-1, also known as Twist-related protein 2, is a basic helix-loop-helix transcription factor that has been shown to be a negative regulator for gene transcription and apoptosis. Basic helix-loop-helix transcription factors are implicated in cell lineage determination and differentiation. Dermo-1 shares extensive homology with another basic helix-loop-helix transcription factor, Twist. Studies suggest that Dermo-1 may inhibit osteoblast maturation during osteoblast development and maintain cells in preosteoblast phenotype.
References
Product Information
FormatAffinity Purified
Control
  • Rat liver tissue lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Dermo-1 Antibody is an antibody against Dermo-1 for use in WB, IH(P).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Dermo-1 in rat liver tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Dermo-1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Rat
  • Chicken
Species Reactivity NoteDemonstrated to react with Human and Rat. Predicted to react with Mouse and Chicken based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryBasic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a ossteoblast phenotype. [provided by RefSeq, Jul 2008].
Gene Symbol
  • TWIST2
  • BHLHA39
  • DERMO1
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Binds to the E-box consensus sequence 5'-CANNTG-3' as a heterodimer and inhibits transcriptional activation by MYOD1, MYOG, MEF2A and MEF2C. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Involved in postnatal glycogen storage and energy metabolism (By similarity). Inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. Ref.1 UniProtKB Q9D030

SUBUNIT STRUCTURE: Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3/E12. Also interacts with MEF2C (By similarity). UniProtKB Q9D030

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Mainly nuclear during embryonic development. Cytoplasmic in adult tissues.

TISSUE SPECIFICITY: In the embryo, highly expressed in chondrogenic cells. In embryonic skin, expressed in the undifferentiated mesenchymal layer beneath the epidermis which later develops into the dermis. Expressed in early myeloid cells but not in lymphoid cells in the liver. Expression also detected in the secretory ependymal epithelium of the choroid plexus primordium. In the adult, expressed in secreting glandular tissues and tubules.

INVOLVEMENT IN DISEASE: Defects in TWIST2 are the cause of Setleis syndrome (SETLEISS) [MIM:227260]. A focal facial dermal dysplasia characterized by distinctive bitemporal scar-like depressions resembling forceps marks, and additional facial features, including a coarse and leonine appearance, absent eyelashes on both lids or multiple rows on the upper lids, absent Meibomian glands, slanted eyebrows, chin clefting, and hypo- or hyperpigmentation of the skin. Histologically, the bitemporal lesion is an ectodermal dysplasia with near absence of subcutaneous fat, suggesting insufficient migration of neural crest cells into the frontonasal process and the first branchial arch.

SEQUENCE SIMILARITIES: Contains 1 basic helix-loop-helix (bHLH) domain.
Molecular Weight~18 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in rat liver tissue lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected Dermo-1 on 10 µg of rat liver tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABD24 04053252533044