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MAB810R-500UG Anti-Cytomegalovirus Antibody, clone 8B1.2

MAB810R-500UG
500 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, FC, IF, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB810R-500UG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Cytomegalovirus Antibody, clone 8B1.2
Alternate Names
  • CMV
Background InformationHuman cytomegalovirus (HCMV) is a ubiquitous human pathogen that belongs to the herpes adenovirus family. The viral life cycle takes approximately seventy two hours. After the initial fusion of the viral envelope with the plasma membrane of the cell, the encapisidated virus particle is released into the cytoplasm and within minutes, transits to the nucleus. Via active transport through the nuclear pore, the capsid gains entry and viral DNA is deposited. Viral gene expression then occurs in a temporally regulated manner, first with expression of the immediate early genes, followed by the early genes, then, after viral replication has commenced, the late genes. All of the immediate early proteins have been shown to be transactivators, with IE1-72 and IE2-86 being the most well characterized. These genes regulate the expression of factors required for virus replication.
References
Product Information
FormatPurified
Control
  • Cytomegalovirus Control Slides, Catalogue Number 5027-5
PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Quality LevelMQ300
Applications
ApplicationAnti-Cytomegalovirus Antibody, clone 8B1.2 is an antibody against Cytomegalovirus for use in ELISA, FC, IF, IH(P) & WB.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesELISA

Western Blot: 1:200. Recognizes approximately a 70kDa band.

Immunofluorescence: 1:200-1:400.

Immunohistochemistry 1:200-1:800.

Works on paraffin embedded tissue sections.

FACS

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenAffinity purified immediate early antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Clone8B1.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with an immediate early non-structural antigen of 68-72 kDa. This antigen can be detected 1 hour after infection exhibiting an intranuclear staining pattern. This staining reaches a peak at 10-12 hours. This antigen persists and can be detected throughout the complete CMV infection cycle.
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Purification MethodProtein A Purfied
Molecular Weight68-72 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Availability by Geography
  • This product is not available for sale in the United States.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size500 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB810R-500UG 04053252610820

Documentation

Anti-Cytomegalovirus Antibody, clone 8B1.2 SDS

Title

Safety Data Sheet (SDS) 

Anti-Cytomegalovirus Antibody, clone 8B1.2 Certificates of Analysis

TitleLot Number
MOUSE ANTI-CYTOMEGALOVIRUS MONOCLONAL ANTIBODY - 2140044 2140044
MOUSE ANTI-CYTOMEGALOVIRUS MONOCLONAL ANTIBODY - 2450186 2450186
MOUSE ANTI-CYTOMEGALOVIRUS MONOCLONAL ANTIBODY - 2455593 2455593
MOUSE ANTI-CYTOMEGALOVIRUS MONOCLONAL ANTIBODY - 2465164 2465164
MOUSE ANTI-CYTOMEGALOVIRUS - 3284344 3284344
MOUSE ANTI-CYTOMEGALOVIRUS - 3503443 3503443
MOUSE ANTI-CYTOMEGALOVIRUS - 3554750 3554750
MOUSE ANTI-CYTOMEGALOVIRUS - 3782110 3782110
MOUSE ANTI-CYTOMEGALOVIRUS - 4185391 4185391
MOUSE ANTI-CYTOMEGALOVIRUS -2607264 2607264

Data Sheet

Title
MOUSE ANTI-CYTOMEGALOVIRUS