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AB5694-I-100UG Anti-ATH 5

Anti-ATH 5, Cat. No. AB5694-I, is a highly specific rabbit polyclonal antibody that targets Protein atonal homolog 7 and has been tested for use in Immunocytochemistry and Western Blotting.

AB5694-I-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberAB5694-I-100UG
DescriptionAnti-ATH 5
Alternate Names
  • Protein atonal homolog 7
  • Class A basic helix-loop-helix protein 13
  • bHLHa13
  • Helix-loop-helix protein hATH-5
Background InformationProtein atonal homolog 7 (UniProt: Q8N100; also known as Class A basic helix-loop-helix protein 13, bHLHa13, Helix-loop-helix protein hATH-5, hATH5) is encoded by the ATOH7 (also known as ATH5, BHLHA13) gene (Gene ID: 220202) in human. ATH5 is a basic helix-loop-helix (bHLH) transcription factor with one bHLH domain (aa 40-92). It is involved in the differentiation of retinal ganglion cells and plays a central role in retinal ganglion cell and optic nerve formation. Overexpression of ATH5 among neurogenic progenitors is shown to significantly enhance the production of retinal ganglionic cells at the expense of reducing the progenitor pool. Higher levels are also reported to accelerate cell cycle progression from S to M phase and promote cell cycle exit. Mutations in ATOH7 gene are known to cause Persistent hyperplastic primary vitreous that is characterized by developmental eye malformation associated with microphthalmia, cataract, glaucoma, and congenital retinal non-attachment. (Ref.: Brown, NL et al., (2002). Mamm. Genome. 13(2); 95-101; Zhang, XM et al., (2018). Sci. Rep. 8; Article number: 6823).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-ATH 5, Cat. No. AB5694-I, is a highly specific rabbit polyclonal antibody that targets Protein atonal homolog 7 and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected ATH 5 in SH-SY5Y cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from within the bHLH domain.
Epitopedomain of:
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityAnti-ATH 5 is a rabbit polyclonal antibody that detects Protein atonal homolog 7 (ATH 5). It targets an epitope with in 19 amino acids from the bHLH domain.
IsotypeIgG
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ATOH7
  • ATH5
  • BHLHA13
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~17 kDa observed; 16.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse retina tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected ATH5 in mouse retina tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5694-I-100UG 04054839007262