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MABC677 Anti-AMACR Antibody, clone 2A10F3

Anti-AMACR Antibody, clone 2A10F3 is a highly specific mouse monoclonal antibody, that targets AMACR & has been tested in western blotting, IHC & ICC.

Anti-AMACR Antibody, clone 2A10F3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: RACE, AMACR UniProt Number: Q9UHK6
MABC677
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, ICCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABC677
DescriptionAnti-AMACR Antibody, clone 2A10F3
Alternate Names
  • Alpha-methylacyl-CoA racemase
  • 2-methylacyl-CoA racemase
Background InformationAlpha-methylacyl-CoA racemase (AMACR) is an enzyme responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their S-stereoisomers. It is a racemase (RACE), which means it will catalyze the stereochemical inversion around the asymmetric carbon atom in a substrate having only one center of asymmetry. AMACR is localized in peroxisomes and mitochondria. Both increases and decreases of the levels of the enzyme have been linked with human diseases. In particular, reduction in AMACR activity or protein levels leads to an increase in bile acids which can result in neurological disorders and Refsum disease, a disorder that ultimately results in cerebellar degeneration and peripheral neuropathy. AMACR deficiency also can lead to peroxisome disorders that lead to the buildup of pristanic acid, DHCA and EHCA which can lead to mental impairment, confusion, leaning difficulties and liver damage. Increased levels of AMACR are associated with prostate cancer and AMACR is widely used as a cancer marker for prostate cancer. In cancer, the protein is called P504S. Finally, AMACR is responsible for the action of ibuprofen; AMACR will convert any R-enantiomer ibuprofen to S-enantiomer, which is the bioactive form that inhibits the activity of the cyclo-oxygenase enzymes and thus bring about the anti-inflammatory effect of the drug. EMD-Millipore’s Anti-AMACR mouse monoclonal antibody, clone 2A10F3, has been tested in western blot against cell lysates from the human prostate adenocarcinoma cell line LNCaP, as well as, Jurkat cells. The clone has also been tested in immunohistochemistry in human prostate, prostate cancer, and normal cerebellum paraffin embedded tissue sections, and in fluorescent immunocytochemistry against LNCaP cells in culture.
References
Product Information
FormatAscites
Control
  • LNCap cell lysate
PresentationMouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-AMACR Antibody, clone 2A10F3 is a highly specific mouse monoclonal antibody, that targets AMACR & has been tested in western blotting, IHC & ICC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected AMACR in human prostate cancer and human kidney tissues.

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected AMACR in LNCaP cells.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified recombinant fragment of human AMACR expressed in E. coli.
Clone2A10F3
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RACE
  • AMACR
Purification MethodUnpurified
UniProt Number
Molecular Weight~45 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 4 isoforms between ~22 kDa and ~44 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in LNCap cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AMACR in 10 µg of LNCap cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC677 04053252910029