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ST1053 Anti-20S Proteasome Core Subunits Rabbit pAb

ST1053
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, YeastRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1053-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes the ~25-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7) in human erythrocytes, HeLa cells and 3T3 cells.
Catalogue NumberST1053
Brand Family Calbiochem®
Application Data
Detection of human 20S proteasome core subunits by immunoblotting. Sample: Whole cell lysate from human erythrocytes. Primary antibody: Anti-20S Proteasome Core Subunits Rabbit pAb (Cat. No. ST1053) (1:1000). Detection: chemiluminescence.
Product Information
FormLiquid
FormulationSerum diluted in PBS.
Positive controlHeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Gong, X., et al. 2007. Cancer Res. 67, 5380.
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (1:1000-1:10,000, see application references)
Immunoprecipitation (see comments)
Paraffin Sections (1:100)
Application CommentsThis antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman erythrocyte proteasomes
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Yeast
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
ST1053-100UL 04055977224382