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17-10501 AccuChIP™ Dimethyl-Histone H3 (Lys27) Chromatin Immunoprecipitation Internal Control

Target-specific spike-in controls that make ChIP experiments more quantitative and accurate. Applications include:
  • Normalization of ChIP signals
  • Normalization of ChIP-seq signals
  • Analysis of antibody specificity

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Chromatin Immunoprecipitation (ChIP), ChIP-seq Research Category: Epigenetics & Nuclear Function
17-10501
100 reactions  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
ChIP, ChIP-seq
Description
Catalogue Number17-10501
Brand Family Upstate
DescriptionAccuChIP™ Dimethyl-Histone H3 (Lys27) Chromatin Immunoprecipitation Internal Control
OverviewAccuChIP™ Chromatin Immunoprecipitation Internal Controls are designed to improve the accuracy of ChIP experiments. This simple to spike-in reagent is composed of a target-specific peptide sequence covalently bound to a uniquely designed double stranded nucleic acid sequence. Because AccuChIP controls mimic the behavior of cross-linked chromatin, by simply adding a known amount of the AccuChIP reagent to your chromatin and performing a standard ChIP reaction followed qPCR detection you can improve the accuracy and reproducibility of your ChIP experiments.

Features and Advantages
  • Simple to use - just add to chromatin either before or after sonication
  • Mimics the behavior of cross-linked chromatin to allow normalization of ChIP signals
  • Target specific control - uses the same antibody used for your ChIP experiment
  • Reduces ChIP variation due to variables such as chromatin preparation, antibody affinity, inconsistent background signals from carry over, and loss of material during handling
  • Easily incorporated into virtually any ChIP protocol
  • Readily detected by qPCR
  • Easily incorporated into ChIP-seq libraries
  • Variety of uniquely designed controls available -add multiple controls to be added to the same chromatin as an process antibody specificity control
Materials Required but Not Delivered
  • Reagents and materials for chromatin isolation from cells or tissues (e.g PureEpi Chromatin Preparation and Optimization kit; EMD Millipore Cat# 17-10082)
  • Sonication device or method to shear chromatin
  • Beads, reagents and buffers to perform chromatin immunoprecipitation (e.g. Magna ChIP HiSens Chromatin Immunoprecipitation Kit EMD Millipore Cat# 17-10460, or EZ-Magna ChIP HiSens Chromatin Immunoprecipitation Kit Cat# 17-10461)
  • ChIP validated antibody or ChIPAb+™ kit
  • IgG negative control antibody (visit www.emdmillipore.com/antibodies to order the IgG negative control antibody used with this control)
  • Real time thermal cycler and qPCR reagents and primers for locus of interest
  • Reagents and instrument for next generation sequencing analysis (optional)
References
Product Information
Components
  • AccuChIP™ H3K27me2 Spike-In Reagent (50 pg/uL )
  • AccuChIP™ H3K27me2 Detection Primer Mix (5 μM)
  • AccuChIP™ Dilution Buffer
PresentationOne box containing three vials (one vial each of target specific AccuChIP™ Spike-In Reagent, target specific AccuChIP™ Detection Primer Mix, and AccuChIP™ Dilution Buffer)
Quality LevelMQ100
Applications
ApplicationTarget-specific spike-in controls that make ChIP experiments more quantitative and accurate. Applications include:
  • Normalization of ChIP signals
  • Normalization of ChIP-seq signals
  • Analysis of antibody specificity
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • ChIP-seq
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsAccuChIP™ Chromatin Immunoprecipitation Internal Controls are stable for 6 months at -20 ºC from date of receipt.
AccuChIP Spike In Reagent is provided as a 20X stock and should be diluted with the provided AccuChIP™ Dilution Buffer to create a working stock. Diluted AccuChIP Spike In Reagent can be stored in aliquots at -20 ºC for up to 3 months. AccuChIP Spike In reagent is composed of a peptide-double stranded DNA conjugate in a buffer containing BSA and glycerol to reduce adherence to the tube. The use of siliconized microfuge tubes for storage of diluted material is recommended.
Packaging Information
Material Size100 reactions
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
17-10501 04055977121643

Documentation

AccuChIP™ Dimethyl-Histone H3 (Lys27) Chromatin Immunoprecipitation Internal Control SDS

Title

Safety Data Sheet (SDS) 

AccuChIP™ Dimethyl-Histone H3 (Lys27) Chromatin Immunoprecipitation Internal Control Certificates of Analysis

TitleLot Number
AccuChIP™ Dimethyl-Histone H3 (Lys27) Chromatin Immunoprecipitation Internal Control - Q2495396 Q2495396

Posters

Title
The use of a synthetic histonepeptide-DNA conjugates as a spike-in reagent to normalize chromatin immunoprecipitation experiments