Amicon® Ultra Centrifugal Filters

Concentration and percent recovery using Amicon® Ultra Filters: 4 different devices (Amicon® Ultra-0.5 mL, Amicon® Ultra-2 mL, Amicon® Ultra-4 mL, Amicon® Ultra-15 mL), were tested with four different proteins (3 kDa Cytochrome C, 10 kDa Cytochrome C, 30 kDa BSA and 100 kDa IgG) to determine percent recovery and concentration factor.

True Dead Stop
The “dead stop” is the volume at which the device will no longer concentrate your sample. This feature:

• Avoids spinning to dryness
• Provides a predictable concentration factor
• No need to calibrate for several samples run in parallel

Reverse Spin Recovery
After you are done concentrating your sample, simply invert it over a new collection tube and spin again to retrieve every drop. This feature provides:

• Maximum protein recovery without introducing pipetting errors
• Over 90% sample recovery, with low-binding membrane and polypropylene housing

Vertical membranes
Unlike some other centrifugal filters where the membrane is horizontal and perpendicular to the direction of filtration, Amicon® Ultra filters feature a vertical membrane, parallel to the direction of filtration. This feature provides:

• Less clogging, less waste and faster filtration
• Ultra-fast sample processing achieving concentration in as little as 10 minutes
• 25- to 80-fold concentration in a single step

Broad Chemical Compatibility
Amicon® Ultra filters are made of materials that are compatible with solutions with diverse chemical properties and pH ranging from 1 to 9.

Also, they are heat-sealed, with no adhesives used to secure the parts of the device to the membrane. This feature minimizes the chance that extractable impurities will be leached into the sample.

Reliable Sample Handling
Spin precious samples with confidence in one robust, sleek unit that prevents leakage.