Millipore Sigma Vibrant Logo
Atención: Nos hemos mudado. Los productos Merck Millipore ya no pueden adquirirse en MerckMillipore.comMás información

71110 rLysozyme™ Solution

Stabilized recombinant lysozyme

rLysozyme™ Solution: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
71110
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
71110-3 Ampolla de plást. 300 ku
71110-4 Ampolla de plást. 1200 ku
71110-5 Ampolla de plást. 6000 ku
Description
OverviewrLysozyme™ Solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of Gram-negative bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction with BugBuster®, BugBuster HT, and PopCulture® Reagents. In the absence of protein extraction reagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27–33 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON® X-100, pH 7.5. rLysozyme Solution is stable at –20°C.
Catalogue Number71110
Brand Family Novagen®
References
Product Information
Unit of DefinitionOne unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A₄₅₀ unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner™(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.
Quality LevelMQ400
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
71110-3 07790788055547
71110-4 04055977254617
71110-5 07790788053048

Documentation

rLysozyme™ Solution Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

rLysozyme™ Solution Certificados de análisis

CargoNúmero de lote
71110

Folleto

Cargo
Bulk Product Guide
Protein Purification and Detection Tools

Licencias necesarias e Información técnica

Cargo
Benzonase®endonuclease for improved primary recovery in an E. coli-based process for Fab production
TB334 rLysozyme Solution

Citas

Título
  • Rachel Balder, et al. (2007) Moraxella catarrhalis strain O35E expresses two filamentous hemagglutinin-like proteins that mediate adherence to human epithelial cells. Infection and Immunity 75, 2765-2775.
  • Benedict M. Long, et al. (2007) Analysis of carboxysomes from Synechococcus PCC7942 reveals multiple rubisco complexes with carboxysomal proteins CcmM and CcaA. Journal of Biological Chemistry 282, 29323-29335.
  • Sehyung Cho, et al. (2005) Glucocorticoid receptor ligand binding domain is sufficient for the modulation of glucocorticoid induction properties by homologous receptors, coactivator transcription intermediary factor 2, and ubc9. Molecular Endocrinology 19, 290-311.
  • S. Moy, et al. (2004) Genome-scale expresion of proteins from Bacillus subtilis. Journal of Structural and Functional Genomics 5, 103-109.
  • Mark A. Rizzo, et al. (2004) An improved cyan fluorescent protein variant useful for FRET. Nature Biotechnology 22, 445-449.
  • B. Husen, et al. (2003) Characterization of 17β-hydroxysteroid dehygronase type 7 in reproductive tissues of the marmoset monkey. Biology of Reproduction 68, 2092-2099.
    		•

    Protocolos de usuario

    Cargo
    TB334 rLysozyme™ Solution