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14-726M c-Kit (D816H) Protein, active, 250 µg

Active, N-terminal GST tagged, recombinant, human c-Kit, amino acids 544-end containing the mutation D816H, for use in Kinase Assays.

14-726M
250 µg  Also available in 10 μg size (cat#14-726) and in bulk (cat#14-726-K).
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-726M
Brand Family Upstate
Trade Name
  • Upstate
Descriptionc-Kit (D816H) Protein, active, 250 µg
OverviewN-terminal GST tagged, recombinant, human c-Kit, amino acids 544-end containing the mutation D816H
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal GST tagged, recombinant, human c-Kit, amino acids 544-end containing the mutation D816H, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 cells
Entrez Gene Number
Entrez Gene SummaryThis gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • KIT
  • C-Kit
  • PBT
  • CD117
  • SCFR
  • c-kit
Protein Targetc-Kit
Purification MethodGlutathione-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P10721 # This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K).
SIZE: 976 amino acids; 109865 Da
SUBUNIT: Interacts with APS. Interacts with the tenth PDZ domain of MPDZ.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P10721 # Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. & Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. & Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT).
SIMILARITY: SwissProt: P10721 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Molecular Weight76.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 250μM substrate peptide (GGMEDIYFEFMGGKKK)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-726) and in bulk (cat#14-726-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-726M 04053252925467

Documentation

Productos y aplicaciones relacionados

Alternative Packsize

Número de referencia Descripción
14-726 c-Kit (D816H) Protein, active, 10 µg

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Categorías

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases