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PF060 TdT, Calf Thymus

PF060
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Descripción

Products

Número de referenciaEmbalaje Cant./Env.
PF060-.155ML Ampolla de plást. .155 ml
Description
OverviewNative TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxy terminus of either double of single stranded DNA in a template-independent manner.
Catalogue NumberPF060
Brand Family Calbiochem®
SynonymsTerminal Deoxynucleotidyl Transferase
References
ReferencesChang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.
Product Information
Unit of DefinitionOne unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3ʹ-OH terminus of d(A)50 in 60 min at 37°C.
FormLiquid
Formulation775 Units in 50 mM potassium phosphate buffer, 1 mM B-mercaptoethanol, 50% glycerol, pH 7.2.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications DNA Labeling
Application NotesDNA Labeling
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Número de referencia GTIN
PF060-.155ML 07790788054007